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World J Gastroenterol. 2017 Dec 7;23(45):8017-8026. doi: 10.3748/wjg.v23.i45.8017.

Pretransplantation fetal-maternal microchimerism in pediatric liver transplantation from mother.

Author information

1
Department of Surgery, Seoul National University College of Medicine, Seoul 03080, South Korea.
2
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul 03080, South Korea.

Abstract

AIM:

To investigate the rates of pretransplantation fetal-maternal microchimerism (MC) and its effect on rejection in children receiving maternal liver grafts.

METHODS:

DNA or blood samples before liver transplantation (LT) were available in 45 pediatric patients and their mothers. The presence of pretransplantation MC to non-inherited maternal antigens (NIMAs) (NIMA-MC) in the peripheral blood was tested using nested PCR-single-strand conformation polymorphism analysis for the human leukocyte antigen (HLA)-DRB1 alleles. NIMA-MC was successfully evaluated in 26 of the 45 children. Among these 45 pediatric LT recipients, 23 children (51.1%) received transplants from maternal donors and the other 22 from non-maternal donors.

RESULTS:

Among these 26 children, pretransplantation NIMA-MC was detected in 23.1% (n = 6), 6.1 (range, 0.8-14) years after birth. Among the children with a maternal donor, the rate of biopsy-proven cellular rejection (BPCR) was 0% in patients with NIMA-MC positivity (0/3) and those with HLA-DR identity with the mother (0/4), but it was 50% in those with NIMA-MC negativity (5/10). Patients with NIMA-MC positivity or HLA-DR identity with the mother showed significantly lower BPCR rate compared with NIMA-MC-negative patients (0% vs 50%, P = 0.04). NIMA-MC-positive patients tended to show lower BPCR rate compared with NIMA-MC-negative patients (P = 0.23).

CONCLUSION:

The presence of pretransplantation NIMA-MC or HLA-DR identity with the mother could be associated with BPCR-free survival in pediatric recipients of LT from maternal donors.

KEYWORDS:

Biopsy-proven cellular rejection; Graft survival; Liver transplantation; Maternal graft; Microchimerism; Non-inherited maternal antigen

PMID:
29259377
PMCID:
PMC5725296
DOI:
10.3748/wjg.v23.i45.8017
[Indexed for MEDLINE]
Free PMC Article

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