Send to

Choose Destination
Biochem J. 2018 Jan 31;475(2):511-529. doi: 10.1042/BCJ20170746.

The hydrophobic region of the Leishmania peroxin 14: requirements for association with a glycosome mimetic membrane.

Author information

Institute of Parasitology, Macdonald Campus of McGill University, Ste-Anne-de-Bellevue, QC, Canada.
Centre for Host-Parasite Interactions, Macdonald Campus of McGill University, Ste-Anne-de-Bellevue, QC, Canada.
Schools of Biology and Chemistry, Biomedical Sciences Research Complex, The North Haugh, The University, St. Andrews, Fife, U.K.
Département d'ophtalmologie, Faculté de Médecine, CUO-Recherche, Centre de recherche du CHU de Québec, Hôpital du Saint-Sacrement, Québec, QC, Canada.
Regroupement stratégique PROTEO, Université Laval, Québec, QC, Canada.
Institute of Parasitology, Macdonald Campus of McGill University, Ste-Anne-de-Bellevue, QC, Canada


Protein import into the Leishmania glycosome requires docking of the cargo-loaded peroxin 5 (PEX5) receptor to the peroxin 14 (PEX14) bound to the glycosome surface. To examine the LdPEX14-membrane interaction, we purified L. donovani promastigote glycosomes and determined the phospholipid and fatty acid composition. These membranes contained predominately phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol (PG) modified primarily with C18 and C22 unsaturated fatty acid. Using large unilamellar vesicles (LUVs) with a lipid composition mimicking the glycosomal membrane in combination with sucrose density centrifugation and fluorescence-activated cell sorting technique, we established that the LdPEX14 membrane-binding activity was dependent on a predicted transmembrane helix found within residues 149-179. Monolayer experiments showed that the incorporation of PG and phospholipids with unsaturated fatty acids, which increase membrane fluidity and favor a liquid expanded phase, facilitated the penetration of LdPEX14 into biological membranes. Moreover, we demonstrated that the binding of LdPEX5 receptor or LdPEX5-PTS1 receptor-cargo complex was contingent on the presence of LdPEX14 at the surface of LUVs.


Leishmania; PEX14; glycosome; membrane proteins; peroxin; transmembrane proteins

[Indexed for MEDLINE]

Publication type, MeSH terms, Substances, Grant support

Publication type

MeSH terms


Grant support

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center