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J Invertebr Pathol. 2018 Mar;153:75-84. doi: 10.1016/j.jip.2017.12.005. Epub 2017 Dec 16.

Pathological analysis of silkworm infected by two microsporidia Nosema bombycis CQ1 and Vairimorpha necatrix BM.

Author information

1
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China.
2
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China; College of Basic Medical Sciences, Zunyi Medical University, Zunyi 563000, PR China.
3
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China. Electronic address: lit@swu.edu.cn.
4
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, PR China; College of Life Sciences, Chongqing Normal University, Chongqing 400047, PR China. Electronic address: zyzhou@swu.edu.cn.

Abstract

Microsporidia Nosema bombycis CQ1 can be vertically transmitted in silkworm Bombyx mori but Vairimorpha necatrix BM cannot. Therefore, the pathological differences in silkworm infected with these two microsporidia required clarification. Here, we compared the virulence of N. bombycis CQ1 and V. necatrix BM against silkworm. The pathological characteristics in intestine, testis and ovary were surveyed using paraffin sections, scanning electron microscopy and transmission electron microscopy. Our data firstly showed that the virulence of V. necatrix BM was weaker than that of N. bombycis CQ1. Secondly, the typical symptom of V. necatrix BM infection is making xenomas, which are full of pathogens in different stages, at the posterior of intestine. However, no xenomas were formed surrounding intestines infected with N. bombycis CQ1. Thirdly, N. bombycis CQ1 can cluster spores near the trachea while infecting ovaries. It is worth noting that N. bombycis CQ1 infected epithelial cells and connective tissues of ovaries, while V. necatrix BM did not. Although silkworm ovaries can not be infected by V. necatrix BM in vivo, it can infect embryonic and ovarian cell lines in vitro. This study is the first report about comparing infection features of N. bombycis CQ1 and V. necatrix BM in silkworm tissues and it provided elaborate and visual information of pathological characteristics which can help to explain the different transmission strategies of these two microsporidia.

KEYWORDS:

Nosema bombycis CQ1; Pathological comparation; Silkworm; VairimorphanecatrixBM; Vertical transmission

PMID:
29258842
DOI:
10.1016/j.jip.2017.12.005

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