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Int J Environ Res Public Health. 2017 Dec 18;14(12). pii: E1587. doi: 10.3390/ijerph14121587.

An Alternative Approach to Investigate Biofilm in Medical Devices: A Feasibility Study.

Author information

1
Science and Technology Park for Medicine, via 29 Maggio 6, 41037 Mirandola, Modena, Italy. tiziana.petrachi@tpm.bio.
2
Department of Medical and Surgical Sciences for Children & Adults, University Hospital of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy. tiziana.petrachi@tpm.bio.
3
Science and Technology Park for Medicine, via 29 Maggio 6, 41037 Mirandola, Modena, Italy. elisa.resca@tpm.bio.
4
Department of Medical and Surgical Sciences for Children & Adults, University Hospital of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy. elisa.resca@tpm.bio.
5
Science and Technology Park for Medicine, via 29 Maggio 6, 41037 Mirandola, Modena, Italy. mariaserena.piccinno@tpm.bio.
6
Department of Medical and Surgical Sciences for Children & Adults, University Hospital of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy. mariaserena.piccinno@tpm.bio.
7
B. Braun Avitum Italy S.p.A., via XXV Luglio 11, 41037 Mirandola, Modena, Italy. francesco.biagi@bbraun.com.
8
Science and Technology Park for Medicine, via 29 Maggio 6, 41037 Mirandola, Modena, Italy. valentina.strusi@tpm.bio.
9
Science and Technology Park for Medicine, via 29 Maggio 6, 41037 Mirandola, Modena, Italy. massimo.dominici@unimore.it.
10
Department of Medical and Surgical Sciences for Children & Adults, University Hospital of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy. massimo.dominici@unimore.it.
11
Science and Technology Park for Medicine, via 29 Maggio 6, 41037 Mirandola, Modena, Italy. elena.veronesi@tpm.bio.
12
Department of Medical and Surgical Sciences for Children & Adults, University Hospital of Modena and Reggio Emilia, via del Pozzo 71, 41124 Modena, Italy. elena.veronesi@tpm.bio.

Abstract

Biofilms are assemblages of bacterial cells irreversibly associated with a surface where moisture is present. In particular, they retain a relevant impact on public health since through biofilms bacteria are able to survive and populate biomedical devices causing severe nosocomial infections that are generally resistant to antimicrobial agents. Therefore, controlling biofilm formation is a mandatory feature during medical device manufacturing and during their use. In this study, combining a crystal violet staining together with advanced stereomicroscopy, we report an alternative rapid protocol for both qualitative and semi-quantitative biofilm determination having high specificity, high repeatability, and low variability. The suggested approach represents a reliable and versatile method to detect, monitor, and measure biofilm colonization by an easy, more affordable, and reproducible method.

KEYWORDS:

bacteria; biofilm; crystal violet; medical device; stereomicroscopy

PMID:
29258219
PMCID:
PMC5751004
DOI:
10.3390/ijerph14121587
[Indexed for MEDLINE]
Free PMC Article

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