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Int J Mol Sci. 2017 Dec 16;18(12). pii: E2736. doi: 10.3390/ijms18122736.

What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo.

Author information

1
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science of Palacký University & Institute of Experimental Botany of the Czech Academy of Sciences, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. barbora.parizkova@upol.cz.
2
Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science of Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. barbora.parizkova@upol.cz.
3
Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science of Palacký University, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. pernisov@sci.muni.cz.
4
Functional Genomics and Proteomics, National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kamenice 5, CZ-62500 Brno, Czech Republic. pernisov@sci.muni.cz.
5
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science of Palacký University & Institute of Experimental Botany of the Czech Academy of Sciences, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic. novako@ueb.cas.cz.

Abstract

Auxins mediate various processes that are involved in plant growth and development in response to specific environmental conditions. Its proper spatio-temporal distribution that is driven by polar auxin transport machinery plays a crucial role in the wide range of auxins physiological effects. Numbers of approaches have been developed to either directly or indirectly monitor auxin distribution in vivo in order to elucidate the basis of its precise regulation. Herein, we provide an updated list of valuable techniques used for monitoring auxins in plants, with their utilities and limitations. Because the spatial and temporal resolutions of the presented approaches are different, their combination may provide a comprehensive outcome of auxin distribution in diverse developmental processes.

KEYWORDS:

auxin; auxin distribution; auxin signalling; auxin transport; direct visualization; indirect visualization; receptor; sensor

PMID:
29258197
PMCID:
PMC5751337
DOI:
10.3390/ijms18122736
[Indexed for MEDLINE]
Free PMC Article

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