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Int J Mol Sci. 2017 Dec 19;18(12). pii: E2754. doi: 10.3390/ijms18122754.

Flavonoids of Kudzu Root Fermented by Eurtotium cristatum Protected Rat Pheochromocytoma Line 12 (PC12) Cells against H₂O₂-Induced Apoptosis.

Author information

1
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. vip_zhangbo87@163.com.
2
College of Food Science and Technology, Bohai University, Jinzhou 121013, China. vip_zhangbo87@163.com.
3
Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou Institute Technology, Xuzhou 221008, China. wenlisony@126.com.
4
College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. dongms@njau.edu.cn.

Abstract

Novel bioactive components have greatly attracted attention as they demonstrate health benefits. Reversed-phase high performance liquid chromatography (RP-HPLC) showed that isoflavonoid compounds of kudzu root (Pueraria lobata) fermented by Eurtotium cristatum and extracted using de-ionized water were higher active compared with non-fermented. A model of H₂O₂-inducd cell damage was built using rat pheochromocytoma line 12 (PC12) cell to observe the protective effect of non-fermented kudzu root (Pueraria lobata) (NFK) and fermented kudzu root (Pueraria lobata) (FK). Cell viability and apoptosis were analyzed through inverted microscopy and flow cytometry. The level of lactate dehydrogenase, catalase activity, superoxide dismutase, glutathione, and reactive oxygen species (ROS) were evaluated. Results showed that NFK and FK could significantly protect PC12 cell against damage caused by H₂O₂-induced oxidative stress. The intracellular antioxidant system was increased, protected the cell membrane inhibit H₂O₂-induced apoptosis by scavenging of ROS. Moreover, NFK and FK regulated the cell cycle to prevent cell apoptosis. Isoflavonoid from the kudzu root especially fermented kudzu root with E. cristatum are potentially therapeutic drugs against diseases induced by oxidative damage.

KEYWORDS:

H2O2-induced damage; PC12 cell; apoptosis; kudzu root (Pueraria lobata); reactive oxygen species

PMID:
29257062
PMCID:
PMC5751353
DOI:
10.3390/ijms18122754
[Indexed for MEDLINE]
Free PMC Article

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