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Nat Methods. 2018 Feb;15(2):115-118. doi: 10.1038/nmeth.4536. Epub 2017 Dec 11.

Mapping the 3D orientation of piconewton integrin traction forces.

Author information

1
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.
2
Department of Chemistry, Emory University, Atlanta, Georgia, USA.
3
Department of Cell Biology, Emory University School of Medicine, Atlanta, Georgia, USA.

Abstract

Mechanical forces are integral to many biological processes; however, current techniques cannot map the magnitude and direction of piconewton molecular forces. Here, we describe molecular force microscopy, leveraging molecular tension probes and fluorescence polarization microscopy to measure the magnitude and 3D orientation of cellular forces. We mapped the orientation of integrin-based traction forces in mouse fibroblasts and human platelets, revealing alignment between the organization of force-bearing structures and their force orientations.

PMID:
29256495
PMCID:
PMC6116908
DOI:
10.1038/nmeth.4536
[Indexed for MEDLINE]
Free PMC Article

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