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Nat Commun. 2017 Dec 18;8(1):2153. doi: 10.1038/s41467-017-01538-9.

Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature.

Author information

1
Deutsches Rheuma-Forschungszentrum, A Leibniz Institute, Charitéplatz 1, 10117, Berlin, Germany.
2
Immune Dynamics, Charité-Universitätsmedizin, Charitéplatz 1, 10117, Berlin, Germany.
3
RISystem AG, Talstraße 2A, 7270, Davos Platz, Switzerland.
4
Department of Immunobiology, Yale University School of Medicine, New Haven, CT, 06519, USA.
5
Julius Wolff Institute, Charité-Universitätsmedizin, Augustenburger Platz 1, 13353, Berlin, Germany.
6
Humboldt-Universität zu Berlin, Unter den Linden 6, 10099, Berlin, Germany.
7
Deutsches Rheuma-Forschungszentrum, A Leibniz Institute, Charitéplatz 1, 10117, Berlin, Germany. hauser@drfz.de.
8
Immune Dynamics, Charité-Universitätsmedizin, Charitéplatz 1, 10117, Berlin, Germany. hauser@drfz.de.
9
Deutsches Rheuma-Forschungszentrum, A Leibniz Institute, Charitéplatz 1, 10117, Berlin, Germany. niesner@drfz.de.

Abstract

The bone marrow is a central organ of the immune system, which hosts complex interactions of bone and immune compartments critical for hematopoiesis, immunological memory, and bone regeneration. Although these processes take place over months, most existing imaging techniques allow us to follow snapshots of only a few hours, at subcellular resolution. Here, we develop a microendoscopic multi-photon imaging approach called LIMB (longitudinal intravital imaging of the bone marrow) to analyze cellular dynamics within the deep marrow. The approach consists of a biocompatible plate surgically fixated to the mouse femur containing a gradient refractive index lens. This microendoscope allows highly resolved imaging, repeatedly at the same regions within marrow tissue, over months. LIMB reveals extensive vascular plasticity during bone healing and steady-state homeostasis. To our knowledge, this vascular plasticity is unique among mammalian tissues, and we expect this insight will decisively change our understanding of essential phenomena occurring within the bone marrow.

PMID:
29255233
PMCID:
PMC5735140
DOI:
10.1038/s41467-017-01538-9
[Indexed for MEDLINE]
Free PMC Article

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