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Sci Rep. 2017 Dec 18;7(1):17740. doi: 10.1038/s41598-017-17062-1.

BCAT1 is a New MR Imaging-related Biomarker for Prognosis Prediction in IDH1-wildtype Glioblastoma Patients.

Author information

1
Department of Radiology, Seoul National University Hospital, Seoul, Korea.
2
Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, Korea.
3
Department of Neurosurgery, Seoul National University Hospital, Seoul, Korea.
4
Department of Pathology, Seoul National University Hospital, Seoul, Korea.
5
Department of Radiology, Seoul National University Hospital, Seoul, Korea. verocay@snuh.org.
6
Center for Nanoparticle Research, Institute for Basic Science (IBS), Seoul, Korea. verocay@snuh.org.

Abstract

Isocitrate dehydrogenase 1 (IDH1)-wildtype glioblastoma (GBM) has found to be accompanied with increased expression of branched-chain amino acid trasaminase1 (BCAT1), which is associated with tumor growth and disease progression. In this retrospective study, quantitative RT-PCR, immunohistochemistry, and western blot were performed with GBM patient tissues to evaluate the BCAT1 level. Quantitative MR imaging parameters were evaluated from DSC perfusion imaging, DWI, contrast-enhanced T1WI and FLAIR imaging using a 3T MR scanner. The level of BCAT1 was significantly higher in IDH1-wildtype patients than in IDH1-mutant patients obtained in immunohistochemistry and western blot. The BCAT1 level was significantly correlated with the mean and 95th percentile-normalized CBV as well as the mean ADC based on FLAIR images. In addition, the 95th percentile-normalized CBV from CE T1WI also had a significant correlation with the BCAT1 level. Moreover, the median PFS in patients with BCAT1 expression <100 was longer than in those with BCAT1 expression ≥100. Taken together, we found that a high BCAT1 level is correlated with high CBV and a low ADC value as well as the poor prognosis of BCAT1 expression is related to the aggressive nature of GBM.

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