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Carbohydr Polym. 2018 Feb 1;181:551-559. doi: 10.1016/j.carbpol.2017.12.002. Epub 2017 Dec 5.

Enzyme functionalized electrospun chitosan mats for antimicrobial treatment.

Author information

1
Empa, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland.
2
Institute of Environmental Biotechnology, BOKU-University of Natural Resources and Life Sciences, Vienna, Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria. Electronic address: gregor.tegl@gmail.com.
3
Empa, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland; École européenne de Chimie, Polymères et Matériaux, Université de Strasbourg, 25 rue Becquerel, 67087 Strasbourg, France.
4
Empa, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014 St. Gallen, Switzerland; CBMN (UMR 5248, CNRS), 3BIO's Team, University of Bordeaux, Allée Geoffroy Saint-Hilaire, 33600 Pessac, France.
5
Empa, Laboratory for Building Energy Materials and Components, Überlandstrasse 129, CH-8600 Dübendorf, Switzerland.
6
Institute of Environmental Biotechnology, BOKU-University of Natural Resources and Life Sciences, Vienna, Konrad Lorenz Straße 20, 3430 Tulln an der Donau, Austria.

Abstract

This work presents electrospun chitosan mats, functionalized with glucose oxidase (GOX) to implement an in-situ hydrogen peroxide (H2O2) generation system. The as spun CTS-PEO mats exhibited a smooth and homogenous morphology in combination with a high specific surface area (5.4m2/g) providing an excellent basis for further functionalization and subsequent glutaraldehyde crosslinking provided them with superior mechanical stability in aqueous environments. GOX was covalently immobilized, as proven by XPS, and resulted in activity recoveries between 20 and 40%. The functional mats generated a steady state concentration of ∼60μM H2O2 per cm2 which resulted in growth inhibition of E. coli and of S. aureus already after two hours of incubation. Additional cytotoxicity tests of the modified mats against mouse fibroblasts did not show an influence on the viability of the cells which proved it a functional biomaterial of great potential for biomedical applications.

KEYWORDS:

Biocompatible; Chitosan; Enzyme immobilization; Glucose oxidase; Hydrogen peroxide; Nanofiber

PMID:
29254006
DOI:
10.1016/j.carbpol.2017.12.002
[Indexed for MEDLINE]

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