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ACS Chem Biol. 2018 Feb 16;13(2):417-423. doi: 10.1021/acschembio.7b00831. Epub 2018 Jan 17.

Protein Inhibitors of CRISPR-Cas9.

Author information

1
Department of Microbiology & Immunology, Quantitative Biosciences Institute, University of California, San Francisco , San Francisco, California 94143, United States.

Abstract

Bacteria are under constant predation from viruses, called bacteriophages (phages). This threat has driven the evolution of multiple defense systems, including the CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR associated genes) immune pathway. Phages are not passive bystanders in their CRISPR-mediated demise, however, as many have developed potent protein inhibitors of the bacterial adaptive immune system. Here, I review the work that led to the discovery of many distinct "anti-CRISPR" proteins. Furthermore, I outline how understanding their mechanisms of action has provided a suite of specific and high-affinity reagents to modulate and study CRISPR-Cas applications.

PMID:
29251498
PMCID:
PMC6049822
DOI:
10.1021/acschembio.7b00831
[Indexed for MEDLINE]
Free PMC Article

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