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J Am Chem Soc. 2018 Jan 31;140(4):1294-1304. doi: 10.1021/jacs.7b08261. Epub 2018 Jan 22.

De Novo Design of Tetranuclear Transition Metal Clusters Stabilized by Hydrogen-Bonded Networks in Helical Bundles.

Author information

1
Department of Chemistry, University of Pennsylvania , 209 South 33rd Street, Philadelphia, Pennsylvania 19104-6396, United States.
2
Department of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California at San Francisco , San Francisco, California 94158-9001, United States.
3
Department of Chemical Sciences, University of Napoli "Federico II" , Via Cintia, 46, 80126 Napoli, Italy.
4
DLX Scientific , Lawrence, Kansas 66049, United States.
5
College of Veterinary Medicine, South China Agricultural University , Guangzhou, Guangdong 510642, China.

Abstract

De novo design provides an attractive approach to test the mechanism by which metalloproteins define the geometry and reactivity of their metal ion cofactors. While there has been considerable progress in designing proteins that bind transition metal ions including iron-sulfur clusters, the design of tetranuclear clusters with oxygen-rich environments has not been accomplished. Here, we describe the design of tetranuclear clusters, consisting of four Zn2+ and four carboxylate oxygens situated at the vertices of a distorted cube-like structure. The tetra-Zn2+ clusters are bound at a buried site within a four-helix bundle, with each helix donating a single carboxylate (Glu or Asp) and imidazole (His) ligand, as well as second- and third-shell ligands. Overall, the designed site consists of four Zn2+ and 16 polar side chains in a fully connected hydrogen-bonded network. The designed proteins have apolar cores at the top and bottom of the bundle, which drive the assembly of the liganding residues near the center of the bundle. The steric bulk of the apolar residues surrounding the binding site was varied to determine how subtle changes in helix-helix packing affect the binding site. The crystal structures of two of four proteins synthesized were in good agreement with the overall design; both formed a distorted cuboidal site stabilized by flanking second- and third-shell interactions that stabilize the primary ligands. A third structure bound a single Zn2+ in an unanticipated geometry, and the fourth bound multiple Zn2+ at multiple sites at partial occupancy. The metal-binding and conformational properties of the helical bundles in solution, probed by circular dichroism spectroscopy, analytical ultracentrifugation, and NMR, were consistent with the crystal structures.

PMID:
29249157
PMCID:
PMC5860638
DOI:
10.1021/jacs.7b08261
[Indexed for MEDLINE]
Free PMC Article

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