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FEBS Lett. 1989 Feb 13;244(1):203-7.

Evidence for two closely related isozymes of arylamine N-acetyltransferase in human liver.

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1
Department of Pharmacology, University of Basel, Switzerland.

Abstract

Acetyl CoA-dependent arylamine N-acetyltransferase (EC 2.3.1.5) is the target of a genetic polymorphism in the metabolism of drugs and carcinogens. N-Acetyltransferase was purified 1000-fold from cytosol of human liver and its identity was verified by amino acid sequence homology of two of its tryptic peptides with published rabbit and chicken N-acetyltransferase sequences. Enzyme activity correlated with the presence of two proteins, NAT-1 and NAT-2, with indistinguishable molecular masses (31 kDa). NAT-1 and NAT-2 could be separated by anion-exchange chromatography and were functionally distinguished by their different apparent affinities for the acceptor amine sulfamethazine (SMZ). Antibodies raised against NAT-1 were able to recognize both isozymes on Western blots.

PMID:
2924904
DOI:
10.1016/0014-5793(89)81193-7
[Indexed for MEDLINE]
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