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J Ethnopharmacol. 2018 Mar 25;214:240-243. doi: 10.1016/j.jep.2017.12.010. Epub 2017 Dec 14.

Reishi mushroom Ganoderma lucidum Modulates IgA production and alpha-defensin expression in the rat small intestine.

Author information

1
Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
2
Department of Pharmacy, Hokkaido University Hospital, Sapporo 060-8648, Japan.
3
Hokkaido Pharmaceutical University School of Pharmacy, Sapporo 006-8590, Japan.
4
Hokkaido Pharmaceutical University School of Pharmacy, Sapporo 006-8590, Japan. Electronic address: natsukot@hokuyakudai.ac.jp.
5
Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan; Department of Pharmacy, Hokkaido University Hospital, Sapporo 060-8648, Japan. Electronic address: ken-i@pharm.hokudai.ac.jp.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Immunoglobulin A (IgA) secretion and alpha-defensins play a role in the innate immune system to protect against infection. Ganoderma lucidum (W.Curt.: Fr.) P. Karst. (Reishi) is a well-known mushroom in traditional Chinese medicine. This study aimed to determine the effects of Reishi on IgA secretion from Peyer's patch (PP) cells and alpha-defensin-5 (RD-5) and RD-6 expression in the rat small intestine.

MATERIALS AND METHODS:

The rats received an oral injection of 0.5-5mg/kg of Reishi powder (1mL/kg) by sonde. All animals were euthanized 24h after Reishi administration. We examined RD-5, RD-6, and Toll-like receptor (TLR) 4 mRNA levels in the jejunum, ileum, and in Peyer's patches (PP) through quantitative real-time PCR analysis. IgA secretion from PP was measured through enzyme-linked immunosorbent assay of the supernatant after primary culture.

RESULTS:

Reishi increased IgA secretion in the presence of lipopolysaccharide (LPS) and increased TLR4 mRNA levels, but had no effect on the viability of PP cells. Moreover, Reishi increased RD-5, RD-6, and TLR4 mRNA levels significantly in the ileum in a concentration-dependent manner.

CONCLUSIONS:

Reishi can induce IgA secretion and increase the mRNA levels of RD-5 and RD-6 in the rat small intestine, through a TLR4-dependent pathway. The present results indicate that Reishi might reduce the risk of intestinal infection.

KEYWORDS:

2.340: ileum; 2.384: lymphocyte; 2.580: traditional Chinese medicine

PMID:
29248453
DOI:
10.1016/j.jep.2017.12.010
[Indexed for MEDLINE]

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