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Development. 2018 Jan 17;145(2). pii: dev158717. doi: 10.1242/dev.158717.

Distinct subsets of Eve-positive pericardial cells stabilise cardiac outflow and contribute to Hox gene-triggered heart morphogenesis in Drosophila.

Author information

1
GReD - INSERM U1103, CNRS UMR6293, University of Clermont Auvergne, 63000 Clermont-Ferrand, France christophe.jagla@uca.fr monika.zmojdzian@uca.fr.
2
GReD - INSERM U1103, CNRS UMR6293, University of Clermont Auvergne, 63000 Clermont-Ferrand, France.

Abstract

The Drosophila heart, composed of discrete subsets of cardioblasts and pericardial cells, undergoes Hox-triggered anterior-posterior morphogenesis, leading to a functional subdivision into heart proper and aorta, with its most anterior part forming a funnel-shaped cardiac outflow. Cardioblasts differentiate into Tin-positive 'working myocytes' and Svp-expressing ostial cells. However, developmental fates and functions of heart-associated pericardial cells remain elusive. Here, we show that the pericardial cells that express the transcription factor Even Skipped adopt distinct fates along the anterior-posterior axis. Among them, the most anterior Antp-Ubx-AbdA-negative cells form a novel cardiac outflow component we call the outflow hanging structure, whereas the Antp-expressing cells differentiate into wing heart precursors. Interestingly, Hox gene expression in the Even Skipped-positive cells not only underlies their antero-posterior diversification, but also influences heart morphogenesis in a non-cell-autonomous way. In brief, we identify a new cardiac outflow component derived from a subset of Even Skipped-expressing cells that stabilises the anterior heart tip, and demonstrate non-cell-autonomous effects of Hox gene expression in the Even Skipped-positive cells on heart morphogenesis.

KEYWORDS:

Drosophila; Heart; Hox genes; Pericardial cell

PMID:
29247145
PMCID:
PMC5825839
DOI:
10.1242/dev.158717
[Indexed for MEDLINE]
Free PMC Article

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