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Pediatr Res. 2018 Mar;83(3):606-614. doi: 10.1038/pr.2017.309. Epub 2018 Jan 17.

RSV vs. rhinovirus bronchiolitis: difference in nasal airway microRNA profiles and NFκB signaling.

Author information

1
Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.
2
Department of Integrative Systems Biology, George Washington University School of Medicine and Health Sciences, Washington, DC.
3
Center for Genetic Medicine Research, Children's National Health System, Washington, DC.
4
Department of Medicine, Boston Children's Hospital, Boston, Massachusetts.
5
Department of Pediatrics, George Washington University School of Medicine and Health Sciences and the Division of Emergency Medicine, Children's National Health System, Washington, DC.
6
Department of Molecular Virology and Microbiology and Pediatrics, Baylor College of Medicine, Houston, Texas.
7
Division of Emergency Medicine, Children's National Health System, Washington, DC.

Abstract

BackgroundAlthough rhinovirus infection is associated with increased risks of acute and chronic respiratory outcomes during childhood compared with respiratory syncytial virus (RSV), the underlying mechanisms remain unclear. We aimed to determine the differences in nasal airway microRNA profiles and their downstream effects between infants with rhinovirus and RSV bronchiolitis.MethodsAs part of a multicenter cohort study of infants hospitalized for bronchiolitis, we examined nasal samples obtained from 16 infants with rhinovirus and 16 infants with RSV. We tested nasal airway samples using microarrays to profile global microRNA expression and determine the predicted regulation of targeted transcripts. We also measured gene expression and cytokines for NFκB pathway components.ResultsBetween the virus groups, 386 microRNAs were differentially expressed (false discovery rate (FDR)<0.05). In infants with rhinovirus, the NFκB pathway was highly ranked as a predicted target for these differentially expressed microRNAs compared with RSV. Pathway analysis using measured mRNA expression data validated that rhinovirus infection had upregulation of NFκB family (RelA and NFκB2) and downregulation of inhibitor κB family. Infants with rhinovirus had higher levels of NFκB-induced type-2 cytokines (IL-10 and IL-13; FDR<0.01).ConclusionIn infants with bronchiolitis, rhinovirus and RSV infections had different nasal airway microRNA profiles associated with NFκB signaling.

PMID:
29244796
PMCID:
PMC6174252
DOI:
10.1038/pr.2017.309
[Indexed for MEDLINE]
Free PMC Article

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