Format

Send to

Choose Destination
Biology (Basel). 2017 Dec 15;6(4). pii: E45. doi: 10.3390/biology6040045.

Exploring the Glycans of Euglena gracilis.

Author information

1
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK. ellis.oneill@plants.ox.ac.uk.
2
Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Sakonwan.Kuhaudomlarp@jic.ac.uk.
3
Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. Martin.Rejzek@jic.ac.uk.
4
Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg 1871, Denmark. Jonatan.Ulrik.Fangel@carlsberg.com.
5
Institute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, Australia. k.alagesan@griffith.edu.au.
6
Department of Biomolecular Sciences, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany. k.alagesan@griffith.edu.au.
7
Institute for Glycomics, Gold Coast Campus, Griffith University, Southport, QLD 4222, Australia. d.kolarich@griffith.edu.au.
8
Department of Biomolecular Sciences, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany. d.kolarich@griffith.edu.au.
9
School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK. William.Willats@newcastle.ac.uk.
10
Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK. rob.field@jic.ac.uk.

Abstract

Euglena gracilis is an alga of great biotechnological interest and extensive metabolic capacity, able to make high levels of bioactive compounds, such as polyunsaturated fatty acids, vitamins and β-glucan. Previous work has shown that Euglena expresses a wide range of carbohydrate-active enzymes, suggesting an unexpectedly high capacity for the synthesis of complex carbohydrates for a single-celled organism. Here, we present an analysis of some of the carbohydrates synthesised by Euglena gracilis. Analysis of the sugar nucleotide pool showed that there are the substrates necessary for synthesis of complex polysaccharides, including the unusual sugar galactofuranose. Lectin- and antibody-based profiling of whole cells and extracted carbohydrates revealed a complex galactan, xylan and aminosugar based surface. Protein N-glycan profiling, however, indicated that just simple high mannose-type glycans are present and that they are partially modified with putative aminoethylphosphonate moieties. Together, these data indicate that Euglena possesses a complex glycan surface, unrelated to plant cell walls, while its protein glycosylation is simple. Taken together, these findings suggest that Euglena gracilis may lend itself to the production of pharmaceutical glycoproteins.

KEYWORDS:

Euglena; N-glycan; algae; biotechnology; carbohydrates; sugar nucleotide

Conflict of interest statement

The authors declare no conflict of interest. The funding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

Supplemental Content

Full text links

Icon for Multidisciplinary Digital Publishing Institute (MDPI) Icon for PubMed Central
Loading ...
Support Center