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Bioanalysis. 2018 Jan;10(1):35-45. doi: 10.4155/bio-2017-0218. Epub 2017 Dec 15.

Assessing a multiplex-targeted proteomics approach for the clinical diagnosis of periodontitis using saliva samples.

Author information

1
U.F.R. d'Odontologie, Département de Parodontologie, 545, avenue du Professeur Jean-Louis Viala, 34 193 Montpellier, Cedex 5.
2
University of Montpellier, LBPC - IRMB, CHU de Montpellier, 80 rue Augustin Fliche, 34 295 Montpellier, France.

Abstract

AIM:

The present study focused on the research of new biomarkers based on the liquid chromatography-multiple-reaction monitoring (MRM) proteomic profile in whole saliva of patients with periodontitis compared with periodontal healthy patients.

METHODS:

A 30-min multiplexed liquid chromatography-MRM method was used for absolute quantification of 35 plasma biomarkers in saliva from control patients and patients with periodontitis.

RESULTS:

Three proteins namely hemopexin, plasminogen and α-fibrinogen were shown to be clearly related to the presence of periodontitis compared with healthy patients. Apolipoprotein H was found to discriminate for the first time chronic and aggressive periodontitis.

CONCLUSION:

Our results indicate that this innovative MRM method could be used to screen for periodontitis in clinical environment. Furthermore, apolipoprotein H was found to be a discriminant biomarker of aggressive periodontitis.

KEYWORDS:

clinical chemistry; mass spectrometry; oral pathology; periodontitis; proteomics; saliva biochemistry

PMID:
29243487
DOI:
10.4155/bio-2017-0218
[Indexed for MEDLINE]

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