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Sci Rep. 2017 Dec 14;7(1):17595. doi: 10.1038/s41598-017-18034-1.

Effects of maternal obesity on Wharton's Jelly mesenchymal stromal cells.

Author information

1
Stem Cell Laboratory, Assisted Conception Unit, School of Life Course Sciences, Department of Women and Children's Health, Faculty of Life Sciences & Medicine, King's College London, London, UK.
2
Genomic Medicine, Houston Methodist Research Institute, Houston, Texas, USA.
3
Department of Haemato-Oncology, Rayne Institute, Faculty of Life Sciences & Medicine, King's College London, London, UK.
4
Histology Laboratory, Wolfson Centre for Age-Related Diseases, Faculty of Life Sciences & Medicine, King's College London, London, UK.
5
Department of Medical and Molecular Genetics, Division of Genetics and Molecular Medicine, King's College London, London, UK.
6
Cardiovascular Division, Faculty of Life Sciences & Medicine, King's College London, London, UK.
7
Stem Cell Laboratory, Assisted Conception Unit, School of Life Course Sciences, Department of Women and Children's Health, Faculty of Life Sciences & Medicine, King's College London, London, UK. dusko.ilic@kcl.ac.uk.

Abstract

We investigated whether maternal metabolic environment affects mesenchymal stromal/stem cells (MSCs) from umbilical cord's Wharton's Jelly (WJ) on a molecular level, and potentially render them unsuitable for clinical use in multiple recipients. In this pilot study on umbilical cords post partum from healthy non-obese (BMI = 19-25; n = 7) and obese (BMI ≥ 30; n = 7) donors undergoing elective Cesarean section, we found that WJ MSC from obese donors showed slower population doubling and a stronger immunosuppressive activity. Genome-wide DNA methylation of triple positive (CD73+CD90+CD105+) WJ MSCs found 67 genes with at least one CpG site where the methylation difference was ≥0.2 in four or more obese donors. Only one gene, PNPLA7, demonstrated significant difference on methylome, transcriptome and protein level. Although the number of analysed donors is limited, our data suggest that the altered metabolic environment related to excessive body weight might bear consequences on the WJ MSCs.

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