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Nat Commun. 2017 Dec 14;8(1):2132. doi: 10.1038/s41467-017-02285-7.

Stochastic gene expression in Arabidopsis thaliana.

Author information

1
Botanical Institute, Biocenter, Cologne University, 50674, Cologne, Germany.
2
Laboratory for Systems and Synthetic Biology, Wageningen University, 6703 HB, Wageningen, The Netherlands.
3
Computational Biology and Biological Physics, Faculty for Theoretical Physics and Astronomy, Lund University, 223 62, Lund, Sweden.
4
Laboratory for Systems and Synthetic Biology, Wageningen University, 6703 HB, Wageningen, The Netherlands. christian.fleck@wur.nl.
5
Botanical Institute, Biocenter, Cologne University, 50674, Cologne, Germany. martin.huelskamp@uni-koeln.de.

Abstract

Although plant development is highly reproducible, some stochasticity exists. This developmental stochasticity may be caused by noisy gene expression. Here we analyze the fluctuation of protein expression in Arabidopsis thaliana. Using the photoconvertible KikGR marker, we show that the protein expressions of individual cells fluctuate over time. A dual reporter system was used to study extrinsic and intrinsic noise of marker gene expression. We report that extrinsic noise is higher than intrinsic noise and that extrinsic noise in stomata is clearly lower in comparison to several other tissues/cell types. Finally, we show that cells are coupled with respect to stochastic protein expression in young leaves, hypocotyls and roots but not in mature leaves. Our data indicate that stochasticity of gene expression can vary between tissues/cell types and that it can be coupled in a non-cell-autonomous manner.

PMID:
29242599
PMCID:
PMC5730595
DOI:
10.1038/s41467-017-02285-7
[Indexed for MEDLINE]
Free PMC Article

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