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PLoS One. 2017 Dec 14;12(12):e0188664. doi: 10.1371/journal.pone.0188664. eCollection 2017.

Microorganisms in the human placenta are associated with altered CpG methylation of immune and inflammation-related genes.

Author information

1
Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, North Carolina, United States of America.
2
Laboratory of Genital Tract Biology, Department of Obstetrics and Gynecology, Harvard Medical School and Brigham and Women's Hospital, Boston, Massachusetts, United States of America.
3
Department of Pathology, Harvard Medical School and Brigham and Women's Hospital, Boston, Massachusetts, United States of America.
4
Division of Pediatric Neurology, Department of Pediatrics, Boston Medical Center, Boston, Massachusetts, United States of America.
5
Department of Pediatrics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina, United States of America.

Abstract

Microorganisms in the placenta have been linked to adverse pregnancy outcomes as well as neonatal illness. Inflammation in the placenta has been identified as a contributing factor in this association, but the underlying biological mechanisms are not yet fully understood. The placental epigenome may serve as an intermediate between placental microbes and inflammation, contributing to adverse outcomes in the offspring. In the present study, genome-wide DNA methylation (n = 486,428 CpG sites) of 84 placentas was analyzed in relation to 16 species of placental microorganisms using samples collected from the Extremely Low Gestation Age Newborns (ELGAN) cohort. A total of n = 1,789 CpG sites, corresponding to n = 1,079 genes, displayed differential methylation (q<0.1) in relation to microorganisms. The altered genes encode for proteins that are involved in immune/inflammatory responses, specifically the NF-κB signaling pathway. These data support bacteria-dependent epigenetic patterning in the placenta and provide potential insight into mechanisms that associate the presence of microorganisms in the placenta to pregnancy and neonatal outcomes. This study lays the foundation for investigations of the placental microbiome and its role in placental function.

PMID:
29240761
PMCID:
PMC5730116
DOI:
10.1371/journal.pone.0188664
[Indexed for MEDLINE]
Free PMC Article

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