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AIDS Res Hum Retroviruses. 2018 Mar;34(3):314-318. doi: 10.1089/AID.2017.0244. Epub 2018 Feb 13.

ARCHITECT HIV Combo Ag/Ab and RealTime HIV-1 Assays Detect Diverse HIV Strains in Clinical Specimens.

Author information

1
1 Infectious Disease Research, Abbott Diagnostics , Abbott Park, Illinois.
2
2 Institut de Recherche en Santé, de Surveillance Epidemiologique et de Formations , Dakar, Senegal .
3
3 Infection Control Unit, King Faisal Specialist Hospital and Research Center , Jeddah, Saudi Arabia .
4
4 Department of Oral and Craniofacial Sciences, School of Dentistry, University of Missouri-Kansas City , Kansas City, Missouri.
5
5 Pathology Department, Truman Medical Center , Kansas City, Missouri.
6
6 Laboratory Research Department, Institute of Human Virology , Abuja, Nigeria .
7
7 Department of Hematology, Université de Yaoundé I , Yaoundé, Cameroon .
8
8 Laboratoire D'Hematologie, Université des Montagnes , Bangangté, Cameroon .

Abstract

Periodic evaluation of the impact of viral diversity on diagnostic tests is critical to ensure current technologies are keeping pace with viral evolution. To determine whether HIV diversity impacts the ARCHITECT HIV Combo Ag/Ab (HIV Combo) or RealTime HIV-1 (RT) assays, a set of N = 199 HIV clinical specimens from Cameroon, Senegal, Saudi Arabia, and Thailand were sequenced and tested in both assays. The panel included historical groups N and P specimens and a newly identified group N specimen. These and specimens classified as H, U (unclassified)/URF (unique recombinant form), CRF (circulating recombinant form) 01, 02, 06, 09, 11, 13, 18, 22, 37, and 43 were detected by both the RT assay (1.75-6.84 log copies/ml) and the HIV Combo assay (3.26-1121.96 sample to cutoff ratios). Sequence alignment identified 3 or fewer mismatches to the RT assay oligos in 82.4% of samples. Altogether, these data demonstrate the HIV Combo and RT assays detect diverse strains of HIV in clinical specimens.

KEYWORDS:

HIV diversity; diagnostics; nucleic acid test; serology

PMID:
29232958
PMCID:
PMC5863103
DOI:
10.1089/AID.2017.0244
[Indexed for MEDLINE]
Free PMC Article

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