Format

Send to

Choose Destination
Elife. 2017 Dec 12;6. pii: e30590. doi: 10.7554/eLife.30590.

A picorna-like virus suppresses the N-end rule pathway to inhibit apoptosis.

Wang Z#1,2, Xia X#1,2,3, Yang X1, Zhang X1,2, Liu Y1,2, Wu D1,2, Fang Y1,2, Liu Y1,2, Xu J2, Qiu Y2, Zhou X1,2.

Author information

1
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
2
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
3
Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology & School of Life Sciences, South China Normal University, Guangzhou, China.
#
Contributed equally

Abstract

The N-end rule pathway is an evolutionarily conserved proteolytic system that degrades proteins containing N-terminal degradation signals called N-degrons, and has emerged as a key regulator of various processes. Viruses manipulate diverse host pathways to facilitate viral replication and evade antiviral defenses. However, it remains unclear if viral infection has any impact on the N-end rule pathway. Here, using a picorna-like virus as a model, we found that viral infection promoted the accumulation of caspase-cleaved Drosophila inhibitor of apoptosis 1 (DIAP1) by inducing the degradation of N-terminal amidohydrolase 1 (NTAN1), a key N-end rule component that identifies N-degron to initiate the process. The virus-induced NTAN1 degradation is independent of polyubiquitylation but dependent on proteasome. Furthermore, the virus-induced N-end rule pathway suppression inhibits apoptosis and benefits viral replication. Thus, our findings demonstrate that a virus can suppress the N-end rule pathway, and uncover a new mechanism for virus to evade apoptosis.

KEYWORDS:

D. melanogaster; Drosophila C virus; N-end rule pathway; apoptosis; immunology; infectious disease; microbiology

PMID:
29231806
PMCID:
PMC5739542
DOI:
10.7554/eLife.30590
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for eLife Sciences Publications, Ltd Icon for PubMed Central
Loading ...
Support Center