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Pharm Biol. 2018 Dec;56(1):12-17. doi: 10.1080/13880209.2017.1413663.

The effect of aqueous extract of Rosa damascena on formaldehyde-induced toxicity in mice testes.

Author information

1
a Physiology Research Center and Department of Physiology , Institute of Neuropharmacology, Kerman University of Medical Sciences , Kerman , Iran.
2
b Department of Anatomy , Kerman University of Medical Sciences , Kerman , Iran.
3
c Department of Physiology , Kurdistan University of Medical Sciences , Sanandaj , Iran.
4
d Research and Clinical Center for Infertility, Yazd Reproductive Institute, Shahid Sadoughi University of Medical Science , Yazd , Iran.
5
e Department of Biochemistry, Afzalipour School of Medicine , Kerman University of Medical Science , Kerman , Iran.
6
f Department of Clinical Biochemistry, School of Pharmacy , Isfahan University of Medical Sciences , Isfahan , Iran.
7
g Department of Anatomy, Afzalipour School of Medicine , Kerman University of Medical Sciences , Kerman , Iran.

Abstract

CONTEXT:

Rosa damascena L. (Rosaceae) (RD) essential oil and extracts are commonly used as a flavour in herbal medicine which increase libido. Previous studies have shown inhalation of RD flower's oil increases libido and causes protective effects in formaldehyde (FA)-induced testicular damage.

OBJECTIVE:

The protective effects of aqueous extract of RD on the male reproductive system of mice were examined following FA-induced damage.

MATERIALS AND METHODS:

Forty-eight adult NMRI male mice were randomly assigned to six groups (n = 8): control (normal saline, 10 mg/kg); RD40 (40 mg/kg, p.o.); FA treated (10 mg/kg of 10%, i.p.) and FA + RD treated at 10, 20 and 40 mg/kg (FA + RD10), (FA + RD20) and (FA + RD40), respectively, for 40 days. At the end of treatment regimes, serum testosterone (T) level and the reproductive activity, viz. body/organ weights, testicular structure and sperm characteristics were studied.

RESULTS:

Formaldehyde administration significantly decreased serum T level (p < 0.001), testicular weight/volume, tubular diameter and sperm characteristics compared to the control group (p < 0.05). RD (40 mg/kg) administration in FA-treated mice significantly improved serum T level, testicular weight/histological structure, tubular diameter, Leydig cell number and epididymal sperm characteristics in comparison to its lower doses and the control group (p < 0.05).

DISCUSSION AND CONCLUSIONS:

We may conclude that RD flower extract can withstand effects of FA in the male reproductive system of mice possibly due to its antioxidative properties.

KEYWORDS:

Formaldehyde toxicity; antioxidant; oxidative stress

PMID:
29231061
PMCID:
PMC6130617
DOI:
10.1080/13880209.2017.1413663
[Indexed for MEDLINE]
Free PMC Article

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