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Stem Cells. 2018 Mar;36(3):313-324. doi: 10.1002/stem.2755. Epub 2017 Dec 25.

A Novel Approach to Single Cell RNA-Sequence Analysis Facilitates In Silico Gene Reporting of Human Pluripotent Stem Cell-Derived Retinal Cell Types.

Author information

1
Waisman Center.
2
McPherson Eye Research Institute.
3
Morgridge Institute for Research, Madison, Wisconsin, USA.
4
Murdoch Children's Research Institute, Parkville, Victoria, Australia.
5
Department of Pediatrics.
6
Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Erratum in

Abstract

Cell type-specific investigations commonly use gene reporters or single-cell analytical techniques. However, reporter line development is arduous and generally limited to a single gene of interest, while single-cell RNA (scRNA)-sequencing (seq) frequently yields equivocal results that preclude definitive cell identification. To examine gene expression profiles of multiple retinal cell types derived from human pluripotent stem cells (hPSCs), we performed scRNA-seq on optic vesicle (OV)-like structures cultured under cGMP-compatible conditions. However, efforts to apply traditional scRNA-seq analytical methods based on unbiased algorithms were unrevealing. Therefore, we developed a simple, versatile, and universally applicable approach that generates gene expression data akin to those obtained from reporter lines. This method ranks single cells by expression level of a bait gene and searches the transcriptome for genes whose cell-to-cell rank order expression most closely matches that of the bait. Moreover, multiple bait genes can be combined to refine datasets. Using this approach, we provide further evidence for the authenticity of hPSC-derived retinal cell types. Stem Cells 2018;36:313-324.

KEYWORDS:

Gene expression profiling; High-throughput RNA sequencing; Pluripotent stem cells; Retina

PMID:
29230913
PMCID:
PMC5823737
DOI:
10.1002/stem.2755
[Indexed for MEDLINE]
Free PMC Article

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