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Exp Parasitol. 2018 Jan;184:90-96. doi: 10.1016/j.exppara.2017.12.001. Epub 2017 Dec 7.

De novo assembly and functional annotations of the transcriptome of Metorchis orientalis (trematoda: Opisthorchiidae).

Author information

1
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China; Department of Parasitology, Heilongjiang Institute of Veterinary Science, Qiqihar, Heilongjiang Province, PR China.
2
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China.
3
College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China. Electronic address: fangming618@126.com.
4
College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China; College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province 163319, PR China. Electronic address: chunrenwang@sohu.com.

Abstract

Metorchis orientalis is a neglected zoonotic parasite, living in the gallbladder and bile duct of poultry and some mammals as well as humans. In spite of its economic and medical importance, the information known about the transcriptome and genome of M. orientalis is limited. In this study, we performed de novo sequencing, transcriptome assembly and functional annotations of the adult M. orientalis, obtained about 77.4 million high-quality clean reads, among which the length of the transcript contigs ranged from 100 to 11,249 nt with mean length of 373 nt and N50 length of 919 nt. We then assembled 31,943 unigenes, of which 20,009 (62.6%) were annotated by BLASTn and BLASTx searches against the available database. Among these unigenes, 19,795 (62.0%), 3407 (10.7%), 10,620 (33.2%) of them had significant similarity in the NR, NT and Swiss-Prot databases, respectively; 5744 (18.0%) and 4678 (14.6%) unigenes were assigned to GO and COG, respectively; and 9099 (28.5%) unigenes were identified and mapped onto 256 pathways in the KEGG Pathway database. Furthermore, we found that 98 (1.08%) unigenes were related to bile secretion and 5 (0.05%) to primary bile acid biosynthesis pathways category. The characterization of these transcriptomic data has implications for the better understanding of the biology of M. orientalis, and will facilitate the development of intervention agents for this and other pathogenic flukes of human and animal health significance.

KEYWORDS:

De novo; Metorchis orientalis; Next-generation sequencing; Transcriptome

PMID:
29225048
DOI:
10.1016/j.exppara.2017.12.001
[Indexed for MEDLINE]

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