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Methods Mol Biol. 2018;1712:217-238. doi: 10.1007/978-1-4939-7514-3_14.

Gene Profiling and T Cell Receptor Sequencing from Antigen-Specific CD4 T Cells.

Author information

1
Department of Immunology and Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA.
2
Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, CA, USA.
3
Department of Immunology and Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA. lteyton@scripps.edu.

Abstract

The paucity of pathogenic T cells in circulating blood limits the information delivered by bulk analysis. Toward diagnosis and monitoring of treatments of autoimmune diseases, we have devised single-cell analysis approaches capable of identifying and characterizing rare circulating CD4 T cells.

KEYWORDS:

Autoimmunity; CD4 T cells; Microfluidics; Peripheral blood; Single-cell analysis; TCR sequences

PMID:
29224077
DOI:
10.1007/978-1-4939-7514-3_14
[Indexed for MEDLINE]

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