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Toxicol In Vitro. 2018 Mar;47:238-248. doi: 10.1016/j.tiv.2017.12.004. Epub 2017 Dec 7.

Biocompatibility of designed MicNo-ZnO particles: Cytotoxicity, genotoxicity and phototoxicity in human skin keratinocyte cells.

Author information

1
Anadolu University, Faculty of Science, Department of Biology, 26470 Eskişehir, Turkey.
2
Anadolu University, Faculty of Science, Department of Biology, 26470 Eskişehir, Turkey. Electronic address: akoparal@anadolu.edu.tr.
3
Anadolu University, Department of Materials Science and Engineering, 26470 Eskisehir, Turkey.
4
Anadolu University, Faculty of Science, Department of Chemistry, 26470 Eskişehir, Turkey.
5
Anadolu University, Department of Materials Science and Engineering, 26470 Eskisehir, Turkey. Electronic address: esuvaci@anadolu.edu.tr.

Abstract

Recently, designed platelet shaped micron particles that are composed of nano primary particles, called MicNo (=Micron+naNo) particles, have been developed to exploit the benefits of nano size, while removing the adverse effects of nanoparticles. It has been shown that MicNo-ZnO particles exhibit both micron and nanosized particle characteristics. Although physical and chemical properties of MicNo-ZnO particles have been studied, their biocompatibility has not yet been evaluated. Accordingly, the research objective of this study was to evaluate in vitro cytotoxicity, genotoxicity and phototoxicity behaviors of designed MicNo-ZnO particles over human epidermal keratinocyte (HaCaT) cells. MicNo-ZnO particles exhibit much less cytotoxicity with IC50 concentrations between 40 and 50μg/ml, genotoxicity above 40μg/ml and lower photo genotoxicity under UVA on HaCaT than the ZnO nanoparticles. Although their chemistries are the same, the source of this difference in toxicity values may be attributed to size differences between the particles that are probably due to their ability to penetrate into the cells. In the present study, the expansive and detailed in vitro toxicity tests show that the biocompatibility of MicNo-ZnO particles is much better than that of the ZnO nanoparticles. Consequently, MicNo-ZnO particles can be considered an important active ingredient alternative for sunscreen applications due to their safer characteristics with respect to ZnO nanoparticles.

KEYWORDS:

Biocompatibility; Cytotoxicity; Genotoxicity; Keratinocyte cells; MicNo-ZnO; Phototoxicity

PMID:
29223573
DOI:
10.1016/j.tiv.2017.12.004
[Indexed for MEDLINE]

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