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J Tissue Eng Regen Med. 2018 Apr;12(4):e1950-e1961. doi: 10.1002/term.2626. Epub 2018 Jan 17.

Bone marrow transplantation improves motor activity in a mouse model of ataxia.

Author information

1
Laboratory of Neuronal Plasticity and Neurorepair, Institute for Neuroscience of Castile and León (INCyL), Universidad de Salamanca, Salamanca, Spain.
2
Institute of Biomedical Research of Salamanca, IBSAL, Salamanca, Spain.
3
Laboratory of Cell Therapy for Neuropathologies, Andalusian Center for Molecular Biology and Regenerative Medicine-CABIMER, CSIC, Seville, Spain.
4
Instituto de Alta Investigación, Universidad de Tarapacá, Arica, Chile.

Abstract

Ataxias are locomotor disorders that can have an origin both neural and muscular, although both impairments are related. Unfortunately, ataxia has no cure, and the current therapies are aimed at motor re-education or muscular reinforcement. Nevertheless, cell therapy is becoming a promising approach to deal with incurable neural diseases, including neuromuscular ataxias. Here, we have used a model of ataxia, the Purkinje Cell Degeneration (PCD) mutant mouse, to study the effect of healthy (wild-type) bone marrow transplantation on the restoration of defective mobility. Bone marrow transplants (from both mutant and healthy donors) were performed in wild-type and PCD mice. Then, a wide battery of behavioural tests was employed to determine possible motor amelioration in mutants. Finally, cerebellum, spinal cord, and muscle were analysed to study the integration of the transplant-derived cells and the origin of the behavioural changes. Our results demonstrated that the transplant of wild-type bone marrow restores the mobility of PCD mice, increasing their capabilities of movement (52-100% of recovery), exploration (20-71% of recovery), speed (35% of recovery), and motor coordination (25% of recovery). Surprisingly, our results showed that bone marrow transplant notably improves the skeletal muscle structure, which is severely damaged in the mutants, rather than ameliorating the central nervous system. Although a multimodal effect of the transplant is not discarded, muscular improvements appear to be the basis of this motor recovery. Furthermore, the results from our study indicate that bone marrow stem cell therapy can be a safe and effective alternative for dealing with movement disorders such as ataxias.

KEYWORDS:

PCD mouse; ataxia; bone marrow transplantation; cerebellum; muscle; stem cells

PMID:
29222849
DOI:
10.1002/term.2626

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