Format

Send to

Choose Destination
Sci Rep. 2017 Dec 8;7(1):17241. doi: 10.1038/s41598-017-17090-x.

Detection of Prions in Blood of Cervids at the Asymptomatic Stage of Chronic Wasting Disease.

Author information

1
Mitchell Center for Alzheimer's disease and Related Brain Disorders, Dept. of Neurology, McGovern School of Medicine University of Texas Health Science Center at Houston, Houston, TX, 77030, USA.
2
Universidad de los Andes, Facultad de Medicina, Av. San Carlos de Apoquindo, 2200, Las Condes, Santiago, Chile.
3
Veterinary Services Cervid Health Program, APHIS, United States Department of Agriculture, Fort Collins, CO, 80526, USA.
4
Mitchell Center for Alzheimer's disease and Related Brain Disorders, Dept. of Neurology, McGovern School of Medicine University of Texas Health Science Center at Houston, Houston, TX, 77030, USA. Rodrigo.MoralesLoyola@uth.tmc.edu.
5
Mitchell Center for Alzheimer's disease and Related Brain Disorders, Dept. of Neurology, McGovern School of Medicine University of Texas Health Science Center at Houston, Houston, TX, 77030, USA. Claudio.Soto@uth.tmc.edu.
6
Universidad de los Andes, Facultad de Medicina, Av. San Carlos de Apoquindo, 2200, Las Condes, Santiago, Chile. Claudio.Soto@uth.tmc.edu.

Abstract

Chronic wasting disease (CWD) is a rapidly spreading prion disorder affecting captive and free-ranging cervids. The zoonotic potential of CWD is unknown, as well as the mechanism for its highly efficient transmission. A top priority to minimize further spreading of this disease and its potential impact on environmental prion contamination is the development of a non-invasive, sensitive, and specific test for ante-mortem detection of infected animals. Here, we optimized the protein misfolding cyclic amplification (PMCA) assay for highly efficient detection of CWD prions in blood samples. Studies were done using a blind panel of 98 field-collected samples of whole blood from codon 96 glycine/glycine, captive white-tailed deer that were analyzed for prion infection post-mortem by immunohistochemistry (IHC). The results showed a sensitivity of 100% in animals with very poor body condition that were IHC-positive in both brain and lymph nodes, 96% in asymptomatic deer IHC-positive in brain and lymph nodes and 53% in animals at early stages of infection that were IHC-positive only in lymph nodes. The overall mean diagnostic sensitivity was 79.3% with 100% specificity. These findings show that PMCA might be useful as a blood test for routine, live animal diagnosis of CWD.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center