Send to

Choose Destination
Am J Transl Res. 2017 Nov 15;9(11):5003-5011. eCollection 2017.

Overexpression of microRNA-145 protects against rat myocardial infarction through targeting PDCD4.

Author information

Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of MedicineShanghai 200080, P.R. China.
Tongji University School of MedicineShanghai, P.R. China.


Myocardial infarction (MI) is a common cardiovascular disease with high mortality. The aim of the present study was to determine the biological role of miR-145 in MI rats and hypoxia-injured cardiomyocytes and to elucidate the potential mechanism. MI rats were induced by left anterior descending artery (LAD) ligation. qRT-PCR and western blot analysis were performed to determine the mRNA and protein levels, respectively. Compared with sham group, miR-145 levels in MI group were significantly decreased. We observed that lentivirus-mediated overexpression of miR-145 significantly improves cardiac function, reduces infarcted tissue size and prevents post-infarction induced apoptosis in rats after MI. Furthermore, PDCD4 was identified as a novel target of miR-145 in cardiomyocytes, and overexpression of PDCD4 could remarkably restore the miR-145-inhibited cardiomyocytes apoptosis and mitochondrial dysfunction after hypoxia injury. Therefore, our study indicated that miR-145/PDCD4 axis might be potential therapeutic targets for the treatment of MI, and its cardioprotective effect may be attributed to a reduction of mitochondria-mediated apoptosis.


Myocardial infarction; PDCD4; apoptosis; left anterior descending; microRNA-145; mitochondria


Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center