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Res Microbiol. 2018 Sep - Oct;169(7-8):442-449. doi: 10.1016/j.resmic.2017.11.004. Epub 2017 Dec 5.

Reconstitution of the activity of RND efflux pumps: a "bottom-up" approach.

Author information

1
Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, CNRS UMR 7099, Institut de Biologie Physico-Chimique (IBPC), Université Paris Diderot, Sorbonne Paris Cité, PSL Research University, Paris, France. Electronic address: dhenesh.puvanendran@ibpc.fr.
2
Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, CNRS UMR 7099, Institut de Biologie Physico-Chimique (IBPC), Université Paris Diderot, Sorbonne Paris Cité, PSL Research University, Paris, France. Electronic address: quentin.cece@ibpc.fr.
3
Laboratoire de Biologie Physico-Chimique des Protéines Membranaires, CNRS UMR 7099, Institut de Biologie Physico-Chimique (IBPC), Université Paris Diderot, Sorbonne Paris Cité, PSL Research University, Paris, France. Electronic address: martin.picard@ibpc.fr.

Abstract

Efflux pumps are systems devoted to the extrusion of noxious compounds. In this review, we discuss the various strategies that have thus far been undertaken for the investigation of efflux pumps after reconstitution into liposomes. It is challenging to uncover mechanisms and dynamics of efflux pumps due to a number of characteristics: their function depends on the correct assembly of three components and they span two adjacent membranes whose lipid compositions are very different. In addition, efflux pumps are active transporters that need energy to work. We present possible lines of improvement for the study of such systems and provide insights into future goals and challenges of efflux pump reconstitution and transport.

KEYWORDS:

Active transport; In vitro transport; Proteoliposomes

PMID:
29217371
DOI:
10.1016/j.resmic.2017.11.004
[Indexed for MEDLINE]

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