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J Pharmacol Sci. 2017 Dec;135(4):141-147. doi: 10.1016/j.jphs.2017.08.007. Epub 2017 Nov 24.

Crocetin attenuates spared nerve injury-induced neuropathic pain in mice.

Author information

1
Department of Anesthesiology, The Second Hospital of Shandong University, 247 Bei Yuan Street, Jinan 250033, China.
2
ICU of LinYi Central Hospital, LinYi 276400, Shandong, China.
3
Department of Anesthesiology, The Second Hospital of Shandong University, 247 Bei Yuan Street, Jinan 250033, China. Electronic address: lzhaoxin@aliyun.com.

Abstract

Crocetin is the main component of saffron and exhibits anti-oxidative and anti-inflammatory effects. Neuroinflammation and oxidative stress have been recognized to play a crucial role in the pathogenesis of neuropathic pain. We investigated the effect of crocetin in a mouse model with neuropathic pain induced by spared nerve injury (SNI). Crocetin was intrathecally perfused at various doses for up to 12 days starting 3 days before the surgery. Behavioral tests were performed to determine pain sensitivity. The concentrations of proinflammatory cytokines tumor necrosis factor (TNF-α) and interleukin-1β (IL-1β) were measured to assess neuroinflammation. In addition, the enzymatic activity of superoxide dismutase (SOD) was measured to reveal the oxidative stress level. We found that repeated treatment with crocetin dose-dependently attenuated mechanical and thermal allodynia in SNI mice. In addition, treatment with high dose of crocetin reduced SNI-induced increase of TNF-α and IL-1β. Crocetin also restored the activity of mitochondrial MnSOD which was reduced in the sciatic nerve and the spinal cord of SNI mice. Collectively, our data demonstrate that crocetin effectively attenuates the neuropathic pain and significantly suppresses oxidative stress and neuroinflammation in the SNI mouse model, supporting the potential of crocetin in the treatment against neuropathic pain.

KEYWORDS:

Crocetin; Neuroinflammation; Neuropathic pain; Oxidative stress; Spared nerve injury

PMID:
29217355
DOI:
10.1016/j.jphs.2017.08.007
[Indexed for MEDLINE]
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