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J Cell Physiol. 2018 Jun;233(6):4907-4918. doi: 10.1002/jcp.26309. Epub 2018 Jan 15.

Photobiomodulation of mesenchymal stem cells encapsulated in an injectable rhBMP4-loaded hydrogel directs hard tissue bioengineering.

Author information

1
Department of Restorative Dentistry, School of Dentistry, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
2
Cell and Molecular Therapy Center (NUCEL/NETCEM), School of Medicine, University of São Paulo, São Paulo, Brazil.
3
Department of Biochemistry, Chemistry Institute, University of São Paulo, São Paulo, Brazil.
4
Department of Restorative Dentistry, School of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
5
Department of Biodentistry, School of Dentistry, Ibirapuera University, São Paulo, Brazil.
6
Department of Stomatology, School of Dentistry, Universidade de São Paulo, São Paulo, Brazil.
7
Natural and Human Science Center, ABC Federal University, Santo André, Brazil.

Abstract

Photobiomodulation (PBM) therapy displays relevant properties for tissue healing and regeneration, which may be of interest for the tissue engineering field. Here, we show that PBM is able to improve cell survival and to interact with recombinant human Bone Morphogenetic Protein 4 (rhBMP4) to direct and accelerate odonto/osteogenic differentiation of dental derived mesenchymal stem cells (MSCs). MSCs were encapsulated in an injectable and thermo-responsive cell carrier (Pluronic® F-127) loaded with rhBMP4 and then photoactivated. PBM improved MSCs self-renewal and survival upon encapsulation in the Pluronic® F-127. In the presence of rhBMP4, cell odonto/osteogenic differentiation was premature and markedly improved in the photoactivated MSCs. An in vivo calvarial critical sized defect model demonstrated significant increase in bone formation after PBM treatment. Finally, a balance in the reactive oxygen species levels may be related to the favorable results of PBM and rhBMP4 association. PBM may act in synergism with rhBMP4 and is a promise candidate to direct and accelerate hard tissue bioengineering.

KEYWORDS:

hydrogel; laser phototherapy; mesenchymal stem cell; photobiomodulation; pluronic® F-127; recombinant human bone morphogenetic protein 4

PMID:
29215714
DOI:
10.1002/jcp.26309

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