Format

Send to

Choose Destination
J R Soc Interface. 2017 Dec;14(137). pii: 20170717. doi: 10.1098/rsif.2017.0717.

Membrane capacitance of thousands of single white blood cells.

Author information

1
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, People's Republic of China.
2
University of Chinese Academy of Sciences, Beijing, People's Republic of China.
3
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan City, Taiwan, Republic of China.
4
Department of Chemical and Materials Engineering, Chang Gung University, Taoyuan City, Taiwan, Republic of China.
5
Department of Cellular and Molecular Biology, Beijing Chest Hospital, Capital Medical University, Beijing, People's Republic of China.
6
Institute of Microelectronics, Chinese Academy of Sciences, Beijing, People's Republic of China.
7
Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City, Taiwan, Republic of China.
8
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, People's Republic of China jbwang@mail.ie.ac.cn.
9
Graduate Institute of Biochemical and Biomedical Engineering, Chang Gung University, Taoyuan City, Taiwan, Republic of China mhwu@mail.cgu.edu.tw.
10
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, People's Republic of China chenjian@mail.ie.ac.cn.

Abstract

As label-free biomarkers, the electrical properties of single cells are widely used for cell type classification and cellular status evaluation. However, as intrinsic cellular electrical markers, previously reported membrane capacitances (e.g. specific membrane capacitance Cspec and total membrane capacitance Cmem) of white blood cells were derived from tens of single cells, lacking statistical significance due to low cell numbers. In this study, white blood cells were first separated into granulocytes and lymphocytes by density gradient centrifugation and were then aspirated through a microfluidic constriction channel to characterize both Cspec and Cmem Thousands of granulocytes (ncell = 3327) and lymphocytes (ncell = 3302) from 10 healthy blood donors were characterized, resulting in Cspec values of 1.95 ± 0.22 µF cm-2 versus 2.39 ± 0.39 µF cm-2 and Cmem values of 6.81 ± 1.09 pF versus 4.63 ± 0.57 pF. Statistically significant differences between granulocytes and lymphocytes were located for both Cspec and Cmem In addition, neural network-based pattern recognition was used to classify white blood cells, producing successful classification rates of 78.1% for Cspec and 91.3% for Cmem, respectively. These results indicate that as intrinsic bioelectrical markers, membrane capacitances may contribute to the classification of white blood cells.

KEYWORDS:

membrane capacitance; microfluidics; single-cell analysis; white blood cells

PMID:
29212758
PMCID:
PMC5746575
DOI:
10.1098/rsif.2017.0717
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Atypon Icon for PubMed Central
Loading ...
Support Center