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Oncotarget. 2017 Jul 26;8(53):90959-90968. doi: 10.18632/oncotarget.19630. eCollection 2017 Oct 31.

PKM2 activation sensitizes cancer cells to growth inhibition by 2-deoxy-D-glucose.

Author information

1
Department of Radiology, Stanford University, Stanford, CA, USA.
2
Current/Present address: Memorial Sloan Kettering Cancer Center, New York, NY, USA.
3
Advanced Imaging Research Center, University of Texas Southwestern Medical Center, Dallas, TX, USA.
4
Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
5
Applied Sciences Laboratory, GE Healthcare, Menlo Park, CA, USA.
6
National Center for Advancing Translational Sciences, NIH, Bethesda, MD, USA.
7
NIH Chemical Genomics Center, Bethesda, MD, USA.

Abstract

Cancer metabolism has emerged as an increasingly attractive target for interfering with tumor growth. Small molecule activators of pyruvate kinase isozyme M2 (PKM2) suppress tumor formation but have an unknown effect on established tumors. We demonstrate that TEPP-46, a PKM2 activator, results in increased glucose consumption, providing the rationale for combining PKM2 activators with the toxic glucose analog, 2-deoxy-D-glucose (2-DG). Combination treatment resulted in reduced viability of a range of cell lines in standard cell culture conditions at concentrations of drugs that had no effect when used alone. This effect was replicated in vivo on established subcutaneous tumors. We further demonstrated the ability to detect acute metabolic differences in combination treatment using hyperpolarized magnetic resonance spectroscopy (MRS). Combination treated tumors displayed a higher pyruvate to lactate 13C-label exchange 2 hr post-treatment. This ability to assess the effect of drugs non-invasively may accelerate the implementation and clinical translation of drugs that target cancer metabolism.

KEYWORDS:

PKM2; cancer metabolism; hyperpolarized MRI; metabolic imaging; molecular imaging

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