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Viruses. 2017 Dec 4;9(12). pii: E371. doi: 10.3390/v9120371.

Characterization of a Novel Bat Adenovirus Isolated from Straw-Colored Fruit Bat (Eidolon helvum).

Author information

1
Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. hogawa@okayama-u.ac.jp.
2
Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. hogawa@okayama-u.ac.jp.
3
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. kajihara@czc.hokudai.ac.jp.
4
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. n-nao@niid.go.jp.
5
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. shigeno@czc.hokudai.ac.jp.
6
Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. daikuke_fujikura@asahikawa-med.ac.jp.
7
Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. mudenda68@yahoo.com.
8
Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. asmweene04@yahoo.com.
9
Provincial Veterinary Office, Department of Veterinary Services, Ministry of Fisheries and Livestock, P.O. Box 70416, Ndola 50100, Zambia. malisheke@yahoo.com.
10
Department of National Parks and Wildlife, Ministry of Tourism and Arts, Private Bag 1, Chilanga 10101, Zambia. davidsquarre@yahoo.co.uk.
11
Department of Virology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan. masao@okayama-u.ac.jp.
12
Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. hidea-hi@czc.hokudai.ac.jp.
13
Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. hidea-hi@czc.hokudai.ac.jp.
14
Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. hidea-hi@czc.hokudai.ac.jp.
15
Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. hidea-hi@czc.hokudai.ac.jp.
16
Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. h-sawa@czc.hokudai.ac.jp.
17
Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. h-sawa@czc.hokudai.ac.jp.
18
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. h-sawa@czc.hokudai.ac.jp.
19
Global Virus Network, 801 W Baltimore St, Baltimore, MD 21201, USA. h-sawa@czc.hokudai.ac.jp.
20
Department of Disease Control, School of Veterinary Medicine, University of Zambia, P.O. Box 32379, Lusaka 10101, Zambia. atakada@czc.hokudai.ac.jp.
21
Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. atakada@czc.hokudai.ac.jp.
22
Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20, W10, Kita-ku, Sapporo 001-0020, Japan. atakada@czc.hokudai.ac.jp.

Abstract

Bats are important reservoirs for emerging zoonotic viruses. For extensive surveys of potential pathogens in straw-colored fruit bats (Eidolon helvum) in Zambia, a total of 107 spleen samples of E. helvum in 2006 were inoculated onto Vero E6 cells. The cell culture inoculated with one of the samples (ZFB06-106) exhibited remarkable cytopathic changes. Based on the ultrastructural property in negative staining and cross-reactivity in immunofluorescence assays, the virus was suspected to be an adenovirus, and tentatively named E. helvum adenovirus 06-106 (EhAdV 06-106). Analysis of the full-length genome of 30,134 bp, determined by next-generation sequencing, showed the presence of 28 open reading frames. Phylogenetic analyses confirmed that EhAdV 06-106 represented a novel bat adenovirus species in the genus Mastadenovirus. The virus shared similar characteristics of low G + C contents with recently isolated members of species Bat mastadenoviruses E, F and G, from which EhAdV 06-106 diverged by more than 15% based on the distance matrix analysis of DNA polymerase amino acid sequences. According to the taxonomic criteria, we propose the tentative new species name "Bat mastadenovirus H". Because EhAdV 06-106 exhibited a wide in vitro cell tropism, the virus might have a potential risk as an emerging virus through cross-species transmission.

KEYWORDS:

Eidolon helvum; Zambia; adenovirus; bat

PMID:
29207524
PMCID:
PMC5744146
DOI:
10.3390/v9120371
[Indexed for MEDLINE]
Free PMC Article

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