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eNeuro. 2017 Nov 30;4(6). pii: ENEURO.0212-17.2017. doi: 10.1523/ENEURO.0212-17.2017. eCollection 2017 Nov-Dec.

Cross-Laboratory Analysis of Brain Cell Type Transcriptomes with Applications to Interpretation of Bulk Tissue Data.

Author information

1
Graduate Program in Bioinformatics, University of British Columbia, Vancouver V6T 1Z4, Canada.
2
Department of Psychiatry, University of British Columbia, Vancouver V6T 2A1, Canada.
3
Michael Smith Laboratories, University of British Columbia, Vancouver V6T 1Z4, Canada.
4
Campbell Family Mental Health Research Institute of CAMH.
5
Department of Psychiatry and the Department of Pharmacology and Toxicology, University of Toronto, Vancouver M5S 1A8, Canada.

Abstract

Establishing the molecular diversity of cell types is crucial for the study of the nervous system. We compiled a cross-laboratory database of mouse brain cell type-specific transcriptomes from 36 major cell types from across the mammalian brain using rigorously curated published data from pooled cell type microarray and single-cell RNA-sequencing (RNA-seq) studies. We used these data to identify cell type-specific marker genes, discovering a substantial number of novel markers, many of which we validated using computational and experimental approaches. We further demonstrate that summarized expression of marker gene sets (MGSs) in bulk tissue data can be used to estimate the relative cell type abundance across samples. To facilitate use of this expanding resource, we provide a user-friendly web interface at www.neuroexpresso.org.

KEYWORDS:

RNA sequencing; cell type; gene expression; marker gene; microarray

PMID:
29204516
PMCID:
PMC5707795
DOI:
10.1523/ENEURO.0212-17.2017
[Indexed for MEDLINE]
Free PMC Article

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