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Nat Neurosci. 2018 Jan;21(1):139-149. doi: 10.1038/s41593-017-0027-7. Epub 2017 Dec 4.

An interactive framework for whole-brain maps at cellular resolution.

Author information

1
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden. furth@cshl.edu.
2
Department of Neuroscience, Scripps Research Institute, Jupiter, FL, USA.
3
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
4
Department of Bioengineering, Stanford University, Stanford, CA, USA.
5
Department of Molecular Medicine, Scripps Research Institute, Jupiter, FL, USA.
6
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden. dinos.meletis@ki.se.

Abstract

To deconstruct the architecture and function of brain circuits, it is necessary to generate maps of neuronal connectivity and activity on a whole-brain scale. New methods now enable large-scale mapping of the mouse brain at cellular and subcellular resolution. We developed a framework to automatically annotate, analyze, visualize and easily share whole-brain data at cellular resolution, based on a scale-invariant, interactive mouse brain atlas. This framework enables connectivity and mapping projects in individual laboratories and across imaging platforms, as well as multiplexed quantitative information on the molecular identity of single neurons. As a proof of concept, we generated a comparative connectivity map of five major neuron types in the corticostriatal circuit, as well as an activity-based map to identify hubs mediating the behavioral effects of cocaine. Thus, this computational framework provides the necessary tools to generate brain maps that integrate data from connectivity, neuron identity and function.

PMID:
29203898
PMCID:
PMC5994773
DOI:
10.1038/s41593-017-0027-7
[Indexed for MEDLINE]
Free PMC Article

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