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Infect Immun. 2018 Feb 20;86(3). pii: e00559-17. doi: 10.1128/IAI.00559-17. Print 2018 Mar.

Use of Proteins Identified through a Functional Genomic Screen To Develop a Protein Subunit Vaccine That Provides Significant Protection against Virulent Streptococcus suis in Pigs.

Author information

1
USDA, ARS, National Animal Disease Center, Ames, Iowa, USA susan.brockmeier@ars.usda.gov.
2
USDA, ARS, National Animal Disease Center, Ames, Iowa, USA.
3
Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom.
4
Section of Paediatrics, Department of Medicine, Imperial College London, London, United Kingdom.
5
The Royal Veterinary College, Hawkshead Campus, Hatfield, Hertfordshire, United Kingdom.
6
Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.

Abstract

Streptococcus suis is a bacterium that is commonly carried in the respiratory tract and that is also one of the most important invasive pathogens of swine, commonly causing meningitis, arthritis, and septicemia. Due to the existence of many serotypes and a wide range of immune evasion capabilities, efficacious vaccines are not readily available. The selection of S. suis protein candidates for inclusion in a vaccine was accomplished by identifying fitness genes through a functional genomics screen and selecting conserved predicted surface-associated proteins. Five candidate proteins were selected for evaluation in a vaccine trial and administered both intranasally and intramuscularly with one of two different adjuvant formulations. Clinical protection was evaluated by subsequent intranasal challenge with virulent S. suis While subunit vaccination with the S. suis proteins induced IgG antibodies to each individual protein and a cellular immune response to the pool of proteins and provided substantial protection from challenge with virulent S. suis, the immune response elicited and the degree of protection were dependent on the parenteral adjuvant given. Subunit vaccination induced IgG reactive against different S. suis serotypes, indicating a potential for cross protection.

KEYWORDS:

Streptococcus suis; adjuvant; functional genomics; subunit; vaccine

PMID:
29203546
PMCID:
PMC5820948
DOI:
10.1128/IAI.00559-17
[Indexed for MEDLINE]
Free PMC Article

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