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Int J Biol Macromol. 2018 Mar;108:635-641. doi: 10.1016/j.ijbiomac.2017.11.178. Epub 2017 Dec 2.

Chitosan produced from Mucorales fungi using agroindustrial by-products and its efficacy to inhibit Colletotrichum species.

Author information

1
Universidade Federal da Paraíba, Centro de Ciências da Saúde, Cidade Universitária, 58.051-900, João Pessoa, Paraíba, Brazil. Electronic address: luciaraquelramosberger@gmail.com.
2
Universidade Federal de Pernambuco, Centro de Ciências da Saúde,Av. Prof. Moraes Rego, n° 1235, Cidade Universitária, 50.670-901, Recife, Pernambuco, Brazil.
3
Universidade Federal da Paraíba, Centro de Ciências da Saúde, Cidade Universitária, 58.051-900, João Pessoa, Paraíba, Brazil.
4
Universidade Federal Rural de Pernambuco,Av. Dom Manoel de Medeiros, Dois Irmãos, 52.171-900, Recife, Pernambuco, Brazil.
5
Universidade Católica Portuguesa, Campus da Asprela, Rua Dr. António Bernardino de Almeida, 4200-072, Porto, Portugal.

Abstract

This study evaluated corn steep liquor (CSL) and papaya peel juice (PPJ) in mixture as substrates for the cultivation (96h, 28°C, pH 5.6, 150rpm) of Mucorales fungi for chitosan production, and determined the growth-inhibitory effect of the fungal chitosan (FuCS) obtained under optimized conditions against phytopathogenic Colletotrichum species. All Mucorales fungi tested were capable of growing in CSL-PPJ medium, showing FuCS production in the range of 5.02 (Fennelomyces heterothalicus SIS 28) - 15.63mg/g (Cunninghamella elegans SIS 41). Highest FuCS production (37.25mg/g) was achieved when C. elegans was cultivated in medium containing 9.43% CSL and 42.5% PPJ. FuCS obtained under these conditions showed a deacetylation degree of 86%, viscosity of 120cP and molecular weight of 4.08×104g/mol. FuCS at 5000, 7500 and 10,000ppm inhibited the growth of all Colletotrichum species tested. FuCS also induced alterations in the morphology of C. fructicola hyphae. CSL-PPJ mixtures are suitable substrates for the cultivation of Mucorales fungi for FuCS production. Chitosan from C. elegans cultivated in CSL-PPJ medium is effective in inhibiting phytopathogenic Colletotrichum species.

KEYWORDS:

Agroindustrial by-products; Antifungal effects; Fungal chitosan

PMID:
29199126
DOI:
10.1016/j.ijbiomac.2017.11.178
[Indexed for MEDLINE]
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