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Mol Metab. 2018 Jan;7:35-44. doi: 10.1016/j.molmet.2017.11.007. Epub 2017 Nov 22.

Mitochondrial fission is associated with UCP1 activity in human brite/beige adipocytes.

Author information

1
Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France. Electronic address: pisani@unice.fr.
2
Inserm, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, Toulouse 31432, France; University of Toulouse, UMR1048, Paul Sabatier University, Toulouse 31432, France.
3
Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France.
4
Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France; Centre Commun de Microscopie Appliquée, Microscopy and Imaging Platform Côte d'Azur, Nice 06108, France.
5
Department of Experimental and Clinical Medicine, University of Ancona (Politecnica Delle Marche), Ancona 60121, Italy; Center of Obesity, University of Ancona (Politecnica Delle Marche)-United Hospitals, Ancona 60121, Italy.
6
Inserm, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, Toulouse 31432, France; University of Toulouse, UMR1048, Paul Sabatier University, Toulouse 31432, France; Toulouse University Hospitals, Department of Clinical Biochemistry, Toulouse 31059, France.
7
Université Côte d'Azur, CNRS, Inserm, iBV, Nice, France. Electronic address: amri@unice.fr.

Abstract

OBJECTIVE:

Thermogenic adipocytes (i.e. brown or brite/beige adipocytes) are able to burn large amounts of lipids and carbohydrates as a result of highly active mitochondria and enhanced uncoupled respiration, due to UCP1 activity. Although mitochondria are the key organelles for this thermogenic function, limited human data are available.

METHODS/RESULTS:

We characterized changes in the mitochondrial function of human brite adipocytes, using hMADS cells as a model of white- to brite-adipocyte conversion. We found that profound molecular modifications were associated with morphological changes in mitochondria. The fission process was partly driven by the DRP1 protein, which also promoted mitochondrial uncoupling.

CONCLUSION:

Our data demonstrate that white-to-brite conversion of human adipocytes relies on molecular, morphological and functional changes in mitochondria, which enable brite/beige cells to carry out thermogenesis.

KEYWORDS:

Brite/beige adipocyte; DRP1; Mitochondria; UCP1; hMADS

PMID:
29198749
PMCID:
PMC5784321
DOI:
10.1016/j.molmet.2017.11.007
[Indexed for MEDLINE]
Free PMC Article

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