Targeted profiling of hydrophilic constituents of royal jelly by hydrophilic interaction liquid chromatography-tandem mass spectrometry

Athanasia Pina; Olga Begou; Dimitris Kanelis; Helen Gika; Stavros Kalogiannis; Chrysoula Tananaki; Georgios Theodoridis; Anastasia Zotou

doi:10.1016/j.chroma.2017.11.019

Targeted profiling of hydrophilic constituents of royal jelly by hydrophilic interaction liquid chromatography-tandem mass spectrometry

J Chromatogr A. 2018 Jan 5:1531:53-63. doi: 10.1016/j.chroma.2017.11.019. Epub 2017 Nov 13.

Authors

Athanasia Pina¹, Olga Begou¹, Dimitris Kanelis², Helen Gika³, Stavros Kalogiannis⁴, Chrysoula Tananaki², Georgios Theodoridis¹, Anastasia Zotou⁵

Affiliations

¹ Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
² Laboratory of Apiculture-Sericulture, School of Agriculture, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece.
³ Laboratory of Forensic Medicine and Toxicology, School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Electronic address: gkikae@auth.gr.
⁴ Department of Nutrition and Dietetics, Alexander Technological Educational Institute (ATEITH) of Thessaloniki, 57400 Thessaloniki, Greece.
⁵ Laboratory of Analytical Chemistry, School of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. Electronic address: azotou@chem.auth.gr.

PMID: 29198446
DOI: 10.1016/j.chroma.2017.11.019

Abstract

In the present work a Hydrophilic Interaction Liquid Chromatography-tandem Mass Spectrometry (HILIC-MS/MS) method was developed for the efficient separation and quantification of a large number of small polar bioactive molecules in Royal Jelly. The method was validated and provided satisfactory detection sensitivity for 88 components. Quantification was proven to be precise for 64 components exhibiting good linearity, recoveries R% >90% for the majority of analytes and intra- and inter-day precision from 0.14 to 20% RSD. Analysis of 125 fresh royal jelly samples of Greek origin provided useful information on royal jelly's hydrophilic bioactive components revealing lysine, ribose, proline, melezitose and glutamic acid to be in high abundance. In addition the occurrence of 18 hydrophilic nutrients which have not been reported previously as royal jelly constituents is shown.

Keywords: Food quality; HILIC; Mass spectrometry; Nutrients; Royal jelly; UHPLC.

MeSH terms

Chromatography, High Pressure Liquid / methods*
Fatty Acids / analysis*
Fatty Acids / chemistry
Fatty Acids / isolation & purification
Food Analysis
Hydrophobic and Hydrophilic Interactions
Limit of Detection
Tandem Mass Spectrometry*

Substances

Fatty Acids
royal jelly