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J Agric Food Chem. 2018 Jan 10;66(1):290-297. doi: 10.1021/acs.jafc.7b03582. Epub 2017 Dec 19.

Multiplexed Detection of Foodborne Pathogens from Contaminated Lettuces Using a Handheld Multistep Lateral Flow Assay Device.

Author information

1
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon 34141, Republic of Korea.
2
Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration , Wanju-gun 55365, Republic of Korea.
3
Department of Agricultural Biotechnology, Seoul National University , Seoul 08826, Republic of Korea.
4
Department of Food Science and Technology, Chonbuk National University , Jeonju 54896, Republic of Korea.

Abstract

This paper presents a handheld device that is capable of simplifying multistep assays to perform sensitive detection of foodborne pathogens. The device is capable of multiplexed detection of Escherichia coli (E. coli) O157:H7, Salmonella Typhimurium (S. Typhimurium), Staphylococcus aureus, and Bacillus cereus. The limit of detection for each bacterium was characterized, and then, the detection of bacteria from contaminated fresh lettuces was demonstrated for two representative foodborne pathogens. We employed a sample pretreatment protocol to recover and concentrate target bacteria from contaminated lettuces, which can detect 1.87 × 104 CFU of E. coli O157:H7 and 1.47 × 104 CFU of S. Typhimurium/1 g of lettuce without an enrichment process. Lastly, we demonstrated that the limit of detection can be reduced to 1 CFU of E. coli O157:H7 and 1 CFU of S. Typhimurium/1 g of lettuce by including a 6 h enrichment of contaminated lettuces in growth media before pretreatment.

KEYWORDS:

contaminated lettuces; foodborne pathogens; handheld device; lateral flow assay; multiplexed detection

PMID:
29198101
DOI:
10.1021/acs.jafc.7b03582
[Indexed for MEDLINE]

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