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J Proteomics. 2018 Feb 20;173:1-11. doi: 10.1016/j.jprot.2017.11.017. Epub 2017 Nov 29.

Label-free quantitative proteomic profiling of colon cancer cells identifies acetyl-CoA carboxylase alpha as antitumor target of Citrus limon-derived nanovesicles.

Author information

1
Dipartimento di Biopatologia e Biotecnologie Mediche, Sezione di Biologia e Genetica, University of Palermo, Palermo, Italy.
2
Dipartimento di Biopatologia e Biotecnologie Mediche, Sezione di Biologia e Genetica, University of Palermo, Palermo, Italy; Dipartimento di Discipline Chirurgiche Oncologiche e Stomatologiche, Ricerca Clinica in Medicina Orale, University of Palermo, Palermo, Italy.
3
Dipartimento di Biopatologia e Biotecnologie Mediche, Sezione di Biologia e Genetica, University of Palermo, Palermo, Italy. Electronic address: simona.fontana@unipa.it.
4
Dipartimento di Biopatologia e Biotecnologie Mediche, Sezione di Biologia e Genetica, University of Palermo, Palermo, Italy; Institute of Biomedicine and Molecular Immunology (IBIM), National Research Council, Palermo, Italy. Electronic address: riccardo.alessandro@unipa.it.

Abstract

We have previously isolated exosome-like nanoparticles from Citrus-limon juice, able to inhibit in vitro and in vivo tumor cell growth. In order to deeply understand the mechanism underlying nanovesicle effects, we performed a proteomic profile of treated colorectal cancer cells. Among the proteins differentially expressed after nanovesicle treatment, we found a significant downregulation of the Acetyl-CoA Carboxylase 1 (ACACA) and we demonstrated that silencing ACACA in cancer cells leads to a reduction of cell growth. Our study proved that the anti-tumor effects of Citrus-limon nanovesicles is partly mediated by lipid metabolism inhibition, in particular via ACACA downregulation.

SIGNIFICANCE:

This study represents the attempt to achieve, by a proteomic approach, a better understanding of the role of lemon nanovesicles in affecting colorectal cancer cell growth.

KEYWORDS:

Acetyl-CoA carboxylase; Citrus-limon nanovesicles; Colorectal cancer; Label-free quantitative proteomic analysis

PMID:
29197582
DOI:
10.1016/j.jprot.2017.11.017
[Indexed for MEDLINE]

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