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BMC Genomics. 2017 Dec 2;18(1):938. doi: 10.1186/s12864-017-4347-5.

Identification and characterization of Prunus persica miRNAs in response to UVB radiation in greenhouse through high-throughput sequencing.

Li S1,2, Shao Z3,4, Fu X1,2, Xiao W1,2, Li L1,2, Chen M1,2, Sun M1,2, Li D5,6, Gao D7,8.

Author information

1
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
2
State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
3
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, People's Republic of China.
4
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, People's Republic of China.
5
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, People's Republic of China. dmli2002@163.com.
6
State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, People's Republic of China. dmli2002@163.com.
7
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, 271018, People's Republic of China. dsgao@sdau.edu.cn.
8
State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, People's Republic of China. dsgao@sdau.edu.cn.

Abstract

BACKGROUND:

MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression of target mRNAs involved in plant growth, development, and abiotic stress. As one of the most important model plants, peach (Prunus persica) has high agricultural significance and nutritional values. It is well adapted to be cultivated in greenhouse in which some auxiliary conditions like temperature, humidity, and UVB etc. are needed to ensure the fruit quality. However, little is known about the genomic information of P. persica under UVB supplement. Transcriptome and expression profiling data for this species are therefore important resources to better understand the biological mechanism of seed development, formation and plant adaptation to environmental change. Using a high-throughput miRNA sequencing, followed by qRT-PCR tests and physiological properties determination, we identified the responsive-miRNAs under low-dose UVB treatment and described the expression pattern and putative function of related miRNAs and target genes in chlorophyll and carbohydrate metabolism.

RESULTS:

A total of 164 known peach miRNAs belonging to 59 miRNA families and 109 putative novel miRNAs were identified. Some of these miRNAs were highly conserved in at least four other plant species. In total, 1794 and 1983 target genes for known and novel miRNAs were predicted, respectively. The differential expression profiles of miRNAs between the control and UVB-supplement group showed that UVB-responsive miRNAs were mainly involved in carbohydrate metabolism and signal transduction. UVB supplement stimulated peach to synthesize more chlorophyll and sugars, which was verified by qRT-PCR tests of related target genes and metabolites' content measurement.

CONCLUSION:

The high-throughput sequencing data provided the most comprehensive miRNAs resource available for peach study. Our results identified a series of differentially expressed miRNAs/target genes that were predicted to be low-dose UVB-responsive. The correlation between transcriptional profiles and metabolites contents in UVB supplement groups gave novel clues for the regulatory mechanism of miRNAs in Prunus. Low-dose UVB supplement could increase the chlorophyll and sugar (sorbitol) contents via miRNA-target genes and therefore improve the fruit quality in protected cultivation of peaches.

KEYWORDS:

Chlorophyll and carbohydrates; High-throughput sequencing; MicroRNA; Prunus persica; UVB

PMID:
29197334
PMCID:
PMC5712094
DOI:
10.1186/s12864-017-4347-5
[Indexed for MEDLINE]
Free PMC Article

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