Format

Send to

Choose Destination
Sci Rep. 2017 Dec 1;7(1):16778. doi: 10.1038/s41598-017-15272-1.

Crumbs, Moesin and Yurt regulate junctional stability and dynamics for a proper morphogenesis of the Drosophila pupal wing epithelium.

Author information

1
Aix-Marseille Université, CNRS UMR 7288, Developmental Biology Institute of Marseille Luminy (IBDM), Marseille, France.
2
UMR CNRS 7232 OOB, Université Pierre et Marie Curie, Banyuls-sur-Mer, France.
3
Centre de Biologie du Développement, CNRS UMR5547, Université Paul Sabatier, Toulouse, France.
4
Aix-Marseille Université, CNRS UMR 7288, Developmental Biology Institute of Marseille Luminy (IBDM), Marseille, France. andre.le-bivic@univ-amu.fr.
5
Laboratory of Biochemistry, La Timone University Hospital, Marseille, France.
6
Aix-Marseille University, UMR MD2 and IRBA, Marseille, France.

Abstract

The Crumbs (Crb) complex is a key epithelial determinant. To understand its role in morphogenesis, we examined its function in the Drosophila pupal wing, an epithelium undergoing hexagonal packing and formation of planar-oriented hairs. Crb distribution is dynamic, being stabilized to the subapical region just before hair formation. Lack of crb or stardust, but not DPatj, affects hexagonal packing and delays hair formation, without impairing epithelial polarities but with increased fluctuations in cell junctions and perimeter length, fragmentation of adherens junctions and the actomyosin cytoskeleton. Crb interacts with Moesin and Yurt, FERM proteins regulating the actomyosin network. We found that Moesin and Yurt distribution at the subapical region depends on Crb. In contrast to previous reports, yurt, but not moesin, mutants phenocopy crb junctional defects. Moreover, while unaffected in crb mutants, cell perimeter increases in yurt mutant cells and decreases in the absence of moesin function. Our data suggest that Crb coordinates proper hexagonal packing and hair formation, by modulating junction integrity via Yurt and stabilizing cell perimeter via both Yurt and Moesin. The Drosophila pupal wing thus appears as a useful system to investigate the functional diversification of the Crb complex during morphogenesis, independently of its role in polarity.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center