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J Nutr Biochem. 2018 Mar;53:72-80. doi: 10.1016/j.jnutbio.2017.10.009. Epub 2017 Nov 2.

Effects of fish oils on ex vivo B-cell responses of obese subjects upon BCR/TLR stimulation: a pilot study.

Author information

1
Department of Biochemistry & Molecular Biology, Brody School of Medicine, East Carolina University; East Carolina Diabetes & Obesity Institute, East Carolina University.
2
East Carolina Diabetes & Obesity Institute, East Carolina University.
3
Organic Technologies.
4
Department of Psychology, East Carolina University.
5
Department of Nutrition Science, East Carolina University.
6
Institute of Aquaculture, University of Stirling, UK.
7
Department of Family Medicine, Brody School of Medicine, East Carolina University.
8
Department of Biochemistry & Molecular Biology, Brody School of Medicine, East Carolina University.
9
Department of Pharmaceutical Sciences, University of Colorado, Denver, CO.
10
Department of Biochemistry & Molecular Biology, Brody School of Medicine, East Carolina University; East Carolina Diabetes & Obesity Institute, East Carolina University; Department of Nutrition, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill. Electronic address: shaikhsa@email.unc.edu.

Abstract

The long-chain n-3 polyunsaturated fatty acids (LC-PUFAs) eicosapentaenoic (EPA) and docosahexaenoic acid (DHA) in fish oil have immunomodulatory properties. B cells are a poorly studied target of EPA/DHA in humans. Therefore, in this pilot study, we tested how n-3 LC-PUFAs influence B-cell responses of obese humans. Obese men and women were assigned to consume four 1-g capsules per day of olive oil (OO, n=12), fish oil (FO, n=12) concentrate or high-DHA-FO concentrate (n=10) for 12 weeks in a parallel design. Relative to baseline, FO (n=9) lowered the percentage of circulating memory and plasma B cells, whereas the other supplements had no effect. There were no postintervention differences between the three supplements. Next, ex vivo B-cell cytokines were assayed after stimulation of Toll-like receptors (TLRs) and/or the B-cell receptor (BCR) to determine if the effects of n-3 LC-PUFAs were pathway-dependent. B-cell IL-10 and TNFα secretion was respectively increased with high DHA-FO (n=10), relative to baseline, with respective TLR9 and TLR9+BCR stimulation. OO (n=12) and FO (n=12) had no influence on B-cell cytokines compared to baseline, and there were no differences in postintervention cytokine levels between treatment groups. Finally, ex vivo antibody levels were assayed with FO (n=7) after TLR9+BCR stimulation. Compared to baseline, FO lowered IgM but not IgG levels accompanied by select modifications to the plasma lipidome. Altogether, the results suggest that n-3 LC-PUFAs could modulate B-cell activity in humans, which will require further testing in a larger cohort.

KEYWORDS:

Antibody levels; B cells; B-cell receptor; Cytokines; Fish oil; Lipidomics; Toll-like receptors

PMID:
29195133
PMCID:
PMC5820214
DOI:
10.1016/j.jnutbio.2017.10.009
[Indexed for MEDLINE]
Free PMC Article

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