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Biotechnol Prog. 2018 Mar;34(2):387-396. doi: 10.1002/btpr.2588. Epub 2017 Dec 7.

Sulfanilic acid-modified chitosan mini-spheres and their application for lysozyme purification from egg white.

Author information

1
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Biotecnología. Junín 956, 1113 Buenos Aires, Argentina.
2
CONICET-Universidad de Buenos Aires, Instituto de Nanobiotecnología (NANOBIOTEC), Junín 956, 1113 Buenos Aires, Argentina.
3
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Química Orgánica, Junín 956 (C1113AAD), Buenos Aires, Argentina.
4
CONICET-Universidad de Buenos Aires, Instituto de Química y Fisicoquímica Biológicas (IQUIFIB), Junín 956 (C1113AAD), Buenos Aires, Argentina.
5
Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Cátedra de Tecnología Farmacéutica II. Junín 956, 1113 Buenos Aires, Argentina.

Abstract

A cation exchange matrix with zwitterionic and multimodal properties was synthesized by a simple reaction sequence coupling sulfanilic acid to a chitosan based support. The novel chromatographic matrix was physico-chemically characterized by ss-NMR and ζ potential, and its chromatographic performance was evaluated for lysozyme purification from diluted egg white. The maximum adsorption capacity, calculated according to Langmuir adsorption isotherm, was 50.07 ± 1.47 mg g-1 while the dissociation constant was 0.074 ± 0.012 mg mL-1 . The process for lysozyme purification from egg white was optimized, with 81.9% yield and a purity degree of 86.5%, according to RP-HPLC analysis. This work shows novel possible applications of chitosan based materials. The simple synthesis reactions combined with the simple mode of use of the chitosan matrix represents a novel method to purify proteins from raw starting materials. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 34:387-396, 2018.

KEYWORDS:

chitosan; chromatographic matrix; egg white lysozyme; multimodal ion exchange chromatography; sulfanilic acid

PMID:
29193855
DOI:
10.1002/btpr.2588
[Indexed for MEDLINE]

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