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Sci Rep. 2017 Nov 30;7(1):16607. doi: 10.1038/s41598-017-16832-1.

Improved Detection of Cytokines Produced by Invariant NKT Cells.

Author information

1
Izmir Biomedicine and Genome Center (IBG), 35340, Balcova, Izmir, Turkey.
2
Department of Medical Biology, Faculty of Medicine, Dokuz Eylul University, 35340, Balcova, Izmir, Turkey.
3
Izmir International Biomedicine and Genome Institute (IBG-Izmir), Dokuz Eylul University, 35340, Balcova, Izmir, Turkey.
4
La Jolla Institute for Allergy and Immunology (LJI), 9420 Athena Circle, La Jolla, CA, 92037, USA.
5
Division of Biological Sciences, University of California San Diego, La Jolla, CA, 92037, USA.
6
Izmir Biomedicine and Genome Center (IBG), 35340, Balcova, Izmir, Turkey. gerhard.wingender@deu.edu.tr.
7
La Jolla Institute for Allergy and Immunology (LJI), 9420 Athena Circle, La Jolla, CA, 92037, USA. gerhard.wingender@deu.edu.tr.

Abstract

Invariant Natural killer T (iNKT) cells rapidly produce copious amounts of multiple cytokines after in vivo activation, allowing for the direct detection of a number of cytokines directly ex vivo. However, for some cytokines this approach is suboptimal. Here, we report technical variations that allow the improved detection of IL-4, IL-10, IL-13 and IL-17A ex vivo. Furthermore, we describe an alternative approach for stimulation of iNKT cells in vitro that allows a significantly improved detection of cytokines produced by iNKT cells. Together, these protocols allow the detection of iNKT cell cytokines ex vivo and in vitro with increased sensitivity.

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